摘要
以紫花苜蓿‘德宝’的下胚轴、叶片、子叶和叶柄为外植体,研究了不同浓度(1.5、2.0、2.5mg/L)2,4-D对各外植体愈伤组织诱导的影响.结果表明:4种不同外植体的愈伤组织发生能力存在显著差异,下胚轴的愈伤组织诱导率最高,达98.5%,显著高于叶片(P<0.05),其他各外植体之间差异不显著.不同浓度2,4-D下各外植体愈伤组织发生也存在显著差异,浓度1.5mg/L和2.0mg/L的2,4-D MS培养基对愈伤组织的诱导效果优于浓度2.5mg/L的2,4-D MS培养基(P<0.05),且有利于愈伤组织的胚性化.另外,2,4-D浓度在1.5～2.5mg/L范围内,低浓度利于叶片和子叶愈伤组织发生,二者在1.5mg/L浓度下的愈伤率可达100%,显著高于2.5mg/L浓度处理,而叶柄和下胚轴的愈伤率在各浓度下差异不显著.
Medicago sativa cv. ＂Derby＂ was employed to study the effects of 2,4 D of different concentrations on callus inducing of hypoeotyls,blade, cotyledon and leaf stem. Results showed that callus proliferation capacity were significantly different among the four types of explants. Callus inducing rate of hypocotyls was up to 98. 5%, which was slightly higher than those of cotyledon and leaf stem, and significantly higher than that of blade （P〈0. 05） ,which was not significantly different with those of cotyledon and leaf stem. In addition,2,4-D of different concentrations had significant effects on callus induction of different explants, 1.5 mg/L 2,4-D ＋ MS medium had the best effectiveness in callus inducing among 4 types of explants（P≤0. 05）,and also contribute to somatic embryogenesis. In the range of 2,4-D from 1.5 mg/L to 2.5 mg/L,lower concentration treatment （1. 5 rag/L） favored callus generation of blade and cotyledon,whose callus treatment. As induction rate bolh reached to 100%, and was significantly higher than that of 2. 5 mg/L. to leaf stem and hypocotyls, there was treatments of 2,4 D no significant difference among different
出处
《甘肃农业大学学报》
CAS
CSCD
北大核心
2012年第4期100-104,共5页
Journal of Gansu Agricultural University
基金
甘肃农业大学草业学院青年教师科研启动项目
关键词
紫花苜蓿
外植体
组织培养
愈伤组织
2
4-D
Medicago sativa
explants
tissue culture
callus
dichlorophenoxyacetic acid （2, 4-D）
