摘要
研究了KCl、NaCl、LiCl、MgCl2、NaNO3、KNO3、Na2SO4、MgSO4 8种不同的电解质对AOT/正己烷反胶束溶液萃取低温花生粕中花生蛋白的影响,对反胶束的含水量、蛋白质的提取率及通过SDS-PAGE电泳试验对蛋白质的亚基条带进行了比较。试验结果表明,电解质的种类会影响反胶束的含水量;阴离子与阳离子对反胶束溶液萃取大豆蛋白的前萃与后萃都有影响,电解质KCl和NaCl溶液所提取的蛋白质得率较高,分别为54.22%和50.19%;不同的电解质可以影响所得蛋白的亚基组成,可以用来分离不同的蛋白。
The review studied on effect of different Na2SO4, MgSO4 ） for extraction of peanut protein in AOT/N salt solutions （ KC1, NaC1, LiC1, MgC12, NaNO3 , KNO3 ,Na2 S04, MgSO4 ）for extraction of peanut protein in AOT/N - hexane reverse micelles from the low - temperature pea-nut meal, and compared with the W0 of the reverse micelles and protein ratio. By SDS - PAGE electrophoresis experi- ment, compared with subunits of protein. The results showed that different salt solutions could cause the changes of W0. The anions and cations could affect the extraction of soybean protein in AOT reverse micelle. In salt solutions, the KC1 and NaC1 solutions in reverse micelles would benefit for protein extraction ,the assignment of protein was 54.22% and 50.19%, respectively. The different salt solutions could cause the subunits of protein,which can be used to sepa- rate different kinds of protein.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2012年第9期76-79,共4页
Journal of the Chinese Cereals and Oils Association
基金
山东省科技发展计划(2011GGC02044)
山东省优秀中青年科学家科研奖励基金(BS2010NY027)
