摘要
利用拟南芥DREB1蛋白序列在水稻全基因组水平进行TBLASTN,共检索到9个水稻同源基因。其中,有3个基因串联排列在第9染色体上,命名为OsCBF1、OsCBF2、OsCBF3。它们编码的蛋白在保守区域内与DREB1的相似性较高。低温驯化能增强水稻幼苗的耐冷性,减少叶片的离子渗漏,但粳稻品种日本晴和籼稻品种93-11之间存在差异。OsCBF1～OsCBF3基因在低温驯化过程中诱导表达,在93-11中的表达量比在日本晴中的更高。CBF候选靶标基因OsP5CS在两个水稻品种都得到诱导表达,但是OsLIP5和OsLIP9只在93-11中上调表达,而在日本晴中表达量变化不明显。OsCBF1～OsCBF3的启动子序列在日本晴与93-11之间存在多态性,93-11的启动子序列中包含更多的MYC顺式作用元件。
Nine CBF/DREB1 homology genes in rice were obtained by BLAST search in the NCBI database, which shares conserved amino acid sequences with DREB1 protein in Arabidopsis. Three CBF genes organized in tandem, named OsCBF1, OsCBF2, OsCBF3, showed a transient induction in the process of cold acclimation, but much stronger in 93-11 compared with that in Nipponbare. The candidate downstream genes OsLIP5 and OsI.IP9 were induced in 93-11 but not in Nipponbare. However, the expression of OsP5CS was induced in both varieties. The differential expression of CBF regulon might be caused by polymorphisms within promoter sequences between these two rice varieties.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2012年第5期521-528,共8页
Chinese Journal of Rice Science
基金
长江中下游突发性灾害应急防控技术研究与集成示范(2012BAD20B05-01-1)
国家现代农业产业技术体系建设专项(nycytx-001)
转基因生物新品种培育科技重点项目(2009ZX08001-009B)
关键词
水稻
低温驯化
基因表达
启动子分析
rice
cold acclimation
gene expression promoter analysis
