摘要
为建立番鸭源小鹅瘟病毒(GPV)快速检测方法,本研究采用GPV单克隆抗体(MAb)标记聚苯乙烯胶乳,建立了检测GPV抗原的胶乳凝集试验(LPA)。结果显示:LPA具有较好的敏感性,可以检出GPV最低毒价为1 000 TCI50/10μL;特异性强,仅与GPV产生特异性凝集反应,与其他相关鸭源病毒均无交叉反应;重复性和稳定性好,在4℃保存期达11个月;对人工感染病例的LPA检测结果与PCR符合率为93.3%;对临床18份疑似GPV病例进行检测,LPA和PCR符合率为94.5%。表明LPA具有简便、快速、特异、准确等优点,适用于基层快速诊断番鸭小鹅瘟病。
To develop the method for goose parvovirus （GPV） detection, the Latex particle agglutination test （LPA） was established by polystyrene latex conjugated with the monoclonal antibody against GPV as diagnostic reagent. The results showed LPA was able to detect GPV for at least 1 000 TCI50 and no cross-reaction with muscovy duck parvovirus, muscovy duck reovirus （MDRV）, novel duck reovirus, duck hepatitis virus, duck pararnyxovirus and avian influenza virus. The storage of the conjugated latex was valid for 11 months at 4 ℃C. The coincidence rate of LPA and PCR was 93.3% for detecting artificial infected duckling and 94.5% for detecting clinical samples. This detection method could be widely used for on-site rapid detection of GPV.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2012年第9期715-718,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
福建省农业科学院创新团队(STIF-Y02)
福建省公益类项目(2009R10025-6)
国家公益类(农业)科研专项经费项目(201003012)
关键词
番鸭小鹅瘟病
单克隆抗体
乳胶凝集技术
muscovy duck gosling plague
monoclonal antibody
latex agglutination assay
