摘要
采用培养皿 ,1承接茶蚜自然分泌蜜露 ;2收集蜜露浓缩后点于培养皿中心。逐头释放瓢虫于分别盛有这 2类蜜露的培养皿中心进行生物测定 ,两个试验均证明 :1蜜露强烈地吸引七星瓢虫和异色瓢虫 ,随着蜜露浓度呈梯度地增大 ,搜寻时间极显著地延长 ( P<0 .0 1 ) ;2蜜露可激发瓢虫的搜索行为由广域搜索型转换为地域集中搜索型。前 0～ 5s局限在较小范围内不断地转向 ,即搜索速度小 ,转动角度大。七星瓢虫和异色瓢虫显明变种最敏感 ,搜索和滞留时间也最长。用高效液相色谱定性定量地分析出蜜露中有茶氨酸等 1 3种氨基酸和蔗糖等 7种糖分。
The bioassay of ladybeetles was carried out on the petri dishes,on which the honeydew of tea aphid was distributed.The ladybeetles were released at the centers of the dishes one by one.Results showed that 2 types of honeydew,i.e.honeydew naturally secreted by tea aphids and honeydew concentrated after secreting,strongly attracted Coccinella septempunctata and 4 varieties of Leis axyridis. The searching and staying time prolonged significantly( P <0.01),with the increasing of the honeydew concentration.Honeydew could induce a switchover of the searching behaviour from area dispersed type to area concentrated type.The slowest searching speeds and the largest bending angles appeared at 0～5 seconds after being released,in which ladybeetles spun within a little area. Coccinella septempunctata and Leis axyridis (Pallas)var. spectabilis were most sensitive among the tested ladybeetles,both searching and staying time were the longest.A chemical analysis on honeydew of Toxoptera aurantii by HPLC showed there were 13 kinds of amino acides and 7 kinds of sugars contained,including sucrose and thea.
出处
《生态学报》
CSCD
北大核心
2000年第3期495-501,共7页
Acta Ecologica Sinica
基金
浙江省自然科学基金课题!(批准号 3 973 0 7)
浙江省分析测试基金课题!(批准号 980 92 )内容之一
关键词
七星瓢虫
异色瓢虫
茶蚜蜜露
搜索行为
化学成分
Coccinella septempunctata L.
Leis axyridis (Pallas)
Tea aphid honeydew
searching behaviour
qualitative and quantitative analysis
