摘要
为了了解小剂量重离子辐射诱导小鼠睾丸结构的适应性反应,采用小剂量(0.05Gy)^(16)O^(8+)离子照射B6C3F_1雄性小鼠睾丸。4h后,再给予2Gy^(16)O^(8+)离子照射。照射后第35天取材在光镜下观察睾丸结构。结果显示,大剂量(2Gy)照射明显损伤睾丸组织,主要表现为曲精细管直径几乎减小一半,精管内各发育阶段的生殖细胞减少或消失,特别是精原细胞几乎完全消失。而Leydig细胞和Sertoli细胞仅有轻度核固缩及胞浆减少。提示睾丸生殖细胞的辐射敏感性明显高于其间质组织细胞。预先给予小剂量(0.05Gy)照射可明显减轻随后大剂量(2Gy)辐射对睾丸组织的损伤。提示小剂量重离子辐射可诱导小鼠睾丸结构明显的适应性反应。
The testes of the B6C3F, hybrid strain mice were irradiated with 0. 05 Gy of 16O8+ ion as the pre-exposure dose (D,) , and were then irradiated with 2 Gy of 16O8+ ion as challenging radiation dose (D2) at 4 h after per-exposure. Testicular morphology was observed by light microscope at 35th day after radiation. The results showed that irradiation of mouse testes with 2 Gy of 16O8+ ion significantly impaired, mainly reduction of tubule diameter and decrease or loss of germ cells in various developing stages, especially spermato-genic elements. Pre-exposure to a low-dose (0. 05 Gy) of 16O8+ ion significantly alleviated above mentioned damage on testicular morphology induced by subsequent a high-dose (2 Gy) radiation.
出处
《实验生物学报》
CSCD
2000年第2期97-100,共4页
Acta Biologiae Experimentalis Sinica
基金
中国博士后科学基金
中国科学院所长基金~~
