摘要
目的:建立双波长高效液相色谱法同时测定双黄连口服液中绿原酸、连翘苷和黄芩苷的不确定度评定方法。方法:根据《测量不确定度评定与表示》(JJF1059-1999)中有关规定,分析影响含量测定结果不确定度的因素来源,对各个不确定度因素进行评定,并计算合成不确定度,最终给出测量结果的扩展不确定度和报告不确定度。结果:不确定度评估为绿原酸±2.23%,连翘苷±9.18%,黄芩苷±0.84%。结论:对照品质量的不确定度是双黄连口服液中3种成分测量不确定度的主要来源。
Objective： To establish a dual-wavelength high performance liquid chromatography method for uncertainty analysis of chlorogenic acid, forsythin and aicalin in Shuanghuanglian Oral Liquid. Method： According to the technical requirements of JJF 1059--1999 （Evaluation and expression of uncertainty in measurement）, each active component of uncertainty was calculated the variable parameters among the procedures. The combined uncertainty and extended uncertainty and reported uncertainty were finally obtained by synthesized the uncertainties of various component variables. Result： Uncertainty evaluation for chlorogenic acid was ± 2.23%, forsythin was ±9.18% and aicalin ±0. 84%. Conclusion： The uncertainty of the reference standard quality was the main source of three components measurement uncertainty in Shuanghuanglian Oral Liquid.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第17期104-108,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江西科技师范大学科研项目(KY2009ZZ04)
