摘要
目的:以原子吸收火焰法测定药用辅料山梨醇中的镍元素,并建立其不确定度评价。方法:药用辅料山梨醇经甲基异丁酮提取后,采用火焰-原子吸收光谱法测定镍元素,并同时对该方法进行不确定度评价。结果:对2个厂家共12批样品进行测定,镍含量分别为0.035 1,0.027 3μg.g-1,RSD 0.54%,定量检测限为0.02 mg.L-1,回收率100.15%,专属性试验表明阴性样品无干扰。供试品溶液及原子吸收光谱仪不确定度贡献率分别为84.01%,15.99%。结论:方法具有操作简单、灵敏度高、重现性好等优点,能满足镍元素的测定要求,为药用辅料山梨醇的质量控制提供依据。
Objective： To determine niccolum in sorbitol with flame-atomic absorption spectroscopy,and set up measurement uncertainty of the method.Method： Niccolum in sorbitol distilled by methylisobutylketone was determined by flame-atomic absorption spectroscopy,and set up measurement uncertainty of the method simultaneously.Result： Nickel content is 0.035 1,0.027 3 μg·g-1 with RSD 0.54%,limit of quantitation is 0.02 mg·L-1,recovery is 100.15%.Specificity test showed no impurity disturbances.The percent of uncertainty of sample solution and atomic absorption spectrometer is 84.01% and 15.99%.Conclusion： This method possesses excellence of simplify manipulation,high veracity and nicer repeatability.It can content the need of niccolum determination and offer basis for quality control of officinal accessories sorbitol.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第20期52-55,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中医药行业科研专项项目(200807032)
关键词
火焰-原子吸收法
山梨醇
镍
不确定度
flame-atomic absorption spectroscopy
sorbitol
niccolum
measurement uncertainty
