摘要
目的:建立HPLC测定豨莶风湿片中粉防己碱含量。方法:采用Diamonsil C18色谱柱,以甲醇-0.3%二乙胺(75∶25)为流动相,检测波长280nm。结果:粉防己碱在0.191 25～1.53μg线性关系良好,r=0.999 8,平均回收率为97.4%(n=5),RSD 1.3%。结论:本法简便、准确、重复性好,可用于豨莶风湿片中粉防己碱的含量测定。
Objective： To establish HPLC for determining the content of tetrandrine in Xixian Fengshi tablet.Method：An HPLC method was developed.The chromatographic conditions were as follow： Diamonsil C18 column and methanol-0.3% diethylamine（75∶25） as mobile phase,detection wavelength at 280 nm.Result：The recovery range of tetrandrine was 0.191 25-1.53 μg,r = 0.999 8.The average recovery rate was 97.4% and RSD was 1.3%.Conclusion： The method is simple and accurate,with good repeatability,and can be used for determination of tetrandrine in Xixian Fengshi tablet.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第2期93-94,共2页
Chinese Journal of Experimental Traditional Medical Formulae
