摘要
24例冠心病患者用党参口服液20ml(含生药党参20g)每日3次,治疗7天;另外10例冠心病患者每日用阿斯匹林0.5g 治疗7天作对照。经治疗,血浆 TXB_2含量在党参组从156.76±11.875pg/ml 明显减至125.01±8.855pg/ml(x±S下同),P<0.05;抑制率为15.67±5.66%,P<0.05;6-酮-PGF_(6-K)含量和抑制率均无明显变化,P>0.05。阿斯匹林组 TBX_2含量也有明显下降,P<0.05,抑制率为38.11±8.69%,P<0.001;6-K 也有较大减少,其抑制率为24.33±9.40%,P<0.05。为了揭示党参对 TXA_2和 PGI_2的作用机制,用制备的猪肺微粒体作为环氧化酶、TXA_2合成酶和 PGI_2合成酶的供酶体,用放免法分别测定了党参对由花生四烯酸或前列腺素内过氧化物合成 TXB_2及6-K 的含量。结果表明,以花生四烯酸或前列腺素内过氧化物合成的 TXB_2含量皆被党参明显抑制;在党参100mg/ml 剂量时仅明显抑制 TXB_2合成,而对6-K 生成无抑制,提示党参在此剂量时呈现 TXA_2合成酶抑制剂作用,当党参剂量为300mg/ml 时,TXA_2和PGI_2的合成酶均被抑制,P<0.001。其作用机理有待进一步研究。
24 angina pectoris patients were treated with Codonopsis pilosulae(CP) oral solution 20 ml (containing crude CP 20 g) thrice daily for 7 days,other 10 cases were treated by aspirin 0.5 g per day for a week as the control group.After treatment,in the CP group,the plasma level of TXB_2 was obviously reduced from 156.76±11.87 pg/ml to 125.01±8.85 pg/ml ((?)±S(?)),the inhibitory rate was 15. 67% (P<0.05),and of 6-keto-PGF_(1α)(6-K) was not markedly changed (P>0.05).In the aspirin group, TXB_2 was also reduced significantly (P<0.05);6-K was reduced more than that of CP group,the inhibitory rate was 24.33±9.40%(P<0.05).To reveal the mechanism of CP action on the synthesis of TXA_2 and PGI_2,the porcine lung microsome was used as the donor of cyclooxygenase,thrombox- ane synthase and prostacyclin synthase,the effects of CP on the formation of TXB_2 and 6-K from arachidonic acid (AA) or endoperoxides were measured by RIA respectively.The results showed that both the levels of the formation of TXB_2 from AA or endoperoxides were markedly reduced by CP in a dose-dependent (at doses of 3～300 mg/ml).The synthesis of TXB_2 was distinctly inhibited alone with a dose of 100 mg/ml CP,which suggested that CP might be an inhibitor of TXB_2 synthase at that dose;while at a dose of 300 mg/ml CP,the synthesis of TXB_2 and 6-K were inhibited simultane- ously (P<0.001).It showed that at a larger dosage of CP,which could inhibited the synthesis of both TXA_2 and PGI_2,its mechanism of action needs further study.
