摘要
目的研究延胡索乙素(L-THP)对大鼠坐骨神经慢性压迫损伤神经病理性疼痛(CCI)的镇痛效应及可能的作用机制。方法雄性Wistar大鼠54只,随机均分为:Sham组(S组)、CCI组(C组)和L-THP组(L组)。S组暴露坐骨神经但不结扎,其余组均建立坐骨神经CCI模型。L组大鼠造模后即刻及术后每日腹腔注射L-THP 40mg/kg,连续14d,S组和C组腹腔注射等量生理盐水。三组大鼠造模前1d、术后3、7、14d测试机械痛阈(MWTs)和热痛阈(TWL),处死取海马组织,用Western blot方法检测脂肪酸酰胺水解酶(FAAH)的表达水平。结果术后3、7、14d三组大鼠术后MWTs和TWL显著低于造模前1d,且C、L组明显低于S组(P<0.05);术后3、7、14d时C、L组大鼠海马FAAH表达水平显著高于S组,且L组FAAH的表达水平显著低于C组(P<0.05)。结论 L-THP对大鼠CCI具有镇痛作用,其机制与抑制海马FAAH的表达相关。
Objective To evaluate the analgesic effect of levo-tetrahydropalmatine （L-THP） in sciatic nerve chronic constriction injury （CCL） induced neuropathic pain. Methods Fifty-four Wistar rats were divided into three groups. Sham group （group S）, CCI group （group C） and L-THP group （group L）. The analgesic effect of L-THP was tested through von Frey filaments and thermal infrared noxious heat stimulator. Western blotting was performed to investigatethe expression of fatty acid amide hydrolase （FAAH） in the hippocampus. Results The threshold mechanical and thermal stimuli at day 3, 7 and 14 were lower than that at day 1, and also both thresholds in groups C and L were higher than those in group S （P〈0.05）. The hippocampal expression 0f FAAH at day 3, 7 and 14 were higher in groups C and L than that of group S, but in group C had upregulated expression of FAAT than in group L （P〈0.05）. Conclusion L-THP produces analgesic effect on neuropathie pain rats by inhibiting the hippocampal expression of FAAT.
出处
《临床麻醉学杂志》
CAS
CSCD
北大核心
2012年第7期705-707,共3页
Journal of Clinical Anesthesiology
关键词
延胡索乙素
神经病理性疼痛
脂肪酸酰胺水解酶
Levo-tetrahydropalmatine
Chronic constriction injury
Fatty acid amide hydrolase
