摘要
利用植物转录因子PTFD数据库1116条陆地棉转录因子DNA序列设计的1455对SSR引物,筛选陆地棉品种/品系渝棉1号、中棉所35、7235和T586,获得66对多态性引物。它们涉及到27个转录因子家族的64个转录因子,其中渝棉1号与中棉所35间23对多态性引物,渝棉1号与T586间30对多态性引物,渝棉1号与7235间33对多态性引物。以多态性引物检测对应重组近交系群体,共获得93个位点。其中,(渝棉1号×中棉所35)群体23个位点,(渝棉1号×T586)群体32个位点,(渝棉1号×7235)群体38个位点。利用转录因子SSR位点与实验室已定位的SSR位点进行遗传连锁分析,将84个位点定位于23条染色体上,其中32个位点分布于A染色体组,52个位点分布于D染色体组。
A total of 1 455 SSR primer pairs designed from 1 116 Gossypium hirsutum transcription factor DNA sequences in Plant Transcription Factor Databases（PTFD） were used to screen the polymorphic primers between upland cotton cultivars/lines Yumian 1,7235,CCRI35,and T586.A total of 66 pairs of polymorphic primers were obtained,which are related to 64 transcription factors among 27 transcription factor families.The polymorphic primers included 23 pairs between yumian1 and CCRI35,30 pairs between Yumian 1 and T586,and 33 pairs between Yumian 1 and 7235.Sixty-six polymorphic primer pairs were used to genotype the corresponding recombinant inbred line populations,and 93 loci were obtained,including 23 loci in population（Yumian 1×CCRI35） F2：6,32 loci in population（Yumian 1×T586） F2：7,and 38 loci in population（Yumian 1×7235） F2：6.The transcription factor SSR loci,together with other SSR loci which have already been mapped on upland cotton linkage map in our laboratory,were used to conduct genetic linkage analysis,and 84 loci were mapped on 23 chromosomes,including 32 loci on A genome and 52 on D genome.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第8期1361-1368,共8页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31071464
30900910)
重庆市自然科学项目(CSTC
2010BB1013)资助
关键词
陆地棉
转录因子
染色体定位
Gossypium hirsutum L.； Transcription factor； Chromosomal localization
