摘要
通过比对拟南芥、金鱼草、水稻和玉米等植物中已知TCP家族转录因子的氨基酸序列,根据保守区设计兼并引物,以陆地棉(Gossypium hirsutum L.)品种新陆早13号的DNA为模板,获得棉花转录因子基因GhTCP1的中间保守区序列。根据该段序列设计特异性引物,采用5'和3'RACE技术获得了全长cDNA序列,编码397个氨基酸。基因组DNA序列分析表明,GhTCP1基因含有一个内含子。棉花GhTCP1蛋白与其它植物中的TCP家族转录因子有较高的相似性,证明该基因在进化过程中是相当保守的。半定量RT-PCR表达分析结果显示,该基因在棉花的侧芽中特异表达。
For isolating the cotton GhTCP1 gene,the amino acid sequences of known TCP genes from other plant species were aligned in order to design degenerate primers based on the conserved amino acid sequence.According to the sequence of the cloned central conserved fragment,specific primers were designed to extend to the 5＇ and 3＇ end through the RACE（rapid amplification cDNA ends） technique to obtain the full length cDNA sequence.The length of open reading frame of GhTCP1 was 1194 bp and encoded 397 amino acids.Sequence alignment indicated that GhTCP1 was clustered in the same group with TCP transcription factors controlling flower symmetry.Semi-quantative RT-PCR analysis indicated that GhTCP1 was specifically expressed in the lateral bud.
出处
《棉花学报》
CSCD
北大核心
2010年第5期409-414,共6页
Cotton Science
基金
国家"转基因生物新品种培育重大专项"-"基因克隆新技术和新方法研究"课题(2008ZX08009-001)
留学人员科技活动择优资助项目"农作物分枝机理及应用研究"
关键词
陆地棉
TCP
基因克隆
序列分析
基因表达
Gossypium hirsutum
TCP
gene clone
sequence analysis
gene expression
