摘要
选择中国工业微生物菌种保藏中心的肠膜明串珠菌20074,通过直接发酵法制备右旋糖酐,以培养液浊度和pH值作为考察指标,对基础培养基的各组分含量进行优化,得到最优组成为:蔗糖10%、蛋白胨0.2%、磷酸氢二钠0.2%、磷酸二氢钾0.03%、酵母浸膏0.5%。实验所选的范围内,金属离子中Mn2+对菌体生长的影响较大。肠膜明串珠菌20074的生长曲线表明,0～8h是生长延迟期,8～24h为对数期,24～30h为稳定期,30h之后为衰亡期。
Dextran had been produced by direct fermentation with Leuconostoc Mesenteroides 20074 from China Industrial Microbiology Culture Collection Center. With turbidity and pH as the index system, the composition of the basal medium for Leuconostoc Mesenteroides 20074 was： 10% sucrose, 0.2% peptone, 0.2% Na2HPO4, 0.03% KH2PO4 and 0.5% yeast extract. Metal ions Mn2~ demonstrated greater impact on cell growth. The growth curve of Leuconostoc Mesenteroides 20074 showed growth delay time is 0-8 h, logarithmic phase 8-24 h, stabilization period 24-30 h, decline phase after 30 h.
出处
《食品科技》
CAS
北大核心
2012年第8期27-31,共5页
Food Science and Technology
基金
广西科学研究与技术开发计划项目(桂科能05112001-5C
桂科能0992028-13)
