摘要
β -环糊精键合硅胶经对甲苯磺酰化后 ,与甘氨酸反应得到甘氨酸衍生化β -环糊精键合硅胶固定相( GCDS)。考察了 GCDS对位置异构体、丹磺酰化氨基酸异构体、苯丙酸类药物等的分离性能 ,研究了流动相中甲醇浓度、p
Glycine derivatized β cyclodextrin bonded silica (GCDS) has been prepared for high performance liquid chromatography through the reactions of β cyclodextrin bonded silica with tosyl chloride and glycine in sequence. The separation performance of GCDS for positional isomers, dansyl amino acids and phenylpropionic acids was investigated. It was found that nitrophenols could not be eluted with methanol as mobile phase, but could be eluted with methanol phosphate buffer solution within appropriate time, which was much different from the behavior observed on native β cyclodextrin bonded silica. The effect of column temperature on the enantio separation of dansyl D,L Phe with GCDS was examined. The results show that the enantio separation ability of the GCDS decreases with the increase of the column temperature. Other factors such as methanol concentration and pH of the mobile phase influencing the retention behavior of solutes on the GCDS are also discussed.
出处
《色谱》
CAS
CSCD
北大核心
2000年第3期224-228,共5页
Chinese Journal of Chromatography
基金
国家自然科学基金资助项目 !( 2 98750 2 0 )
留学回国人员资助费资助项目
关键词
键合硅胶固定相
Β-环糊精
甘氨酸
HPLC
high performance liquid chromatography
bonded silica stationary phase
β cyclodextrin
glycine
