摘要
[Objective] The aim was to carry out isozyme analysis of jin silver carp (Hypophthalmichthys molitrix Var Jing). [Method] The isozyme of AAT, EST, α-GPD, GPI, IDH, LDH, MDH, ME, PGM and PROT of muscles and liver in two populations of the silver carp (Hypophthalmichthys molitrix): Jin silver carp (a breed through selective breeding) and artificially propagated population bought from Jingzhou city, Hubei Province were examined by horizontal starch gel electrophoresis. [Result] Eighteen loci were observed in two populations. Two loci of GPI* and PGM* in Jing silver carp population and the locus of GPI* in Jingzhou population were polymorphic. The proportions of polymorphic loci (maximum allele frequency≤0.99) of Jing silver carp and Jingzhou populations were 11.11% and 5.56% respectively, expected heterozygosity were 0.015 0 and 0.001 1 respectively. The Nei’s genetic distances were 0.000 59 between two populations. The result of chi-square test of the GPI* gene in two populations showed that their genetic structure has very significant difference. [Conclusion] This study provided a theoretical basis for large-scale extension of Jing silver carp.
[目的]对津鲢进行同工酶分析。[方法]使用水平式淀粉凝胶电泳法,对选育品种津鲢进行AAT、EST、α-GPD、GPI、IDH、LDH、MDH、ME、PGM和PROT共10种同工酶及蛋白质的电泳分析,并以购自湖北省荆州市的鲢人工繁殖群体进行比较。[结果]以肌肉和肝脏作为检测用组织,共检测出18个基因座位;在津鲢群体中有变异的基因座位为GPI*和PGM*,荆州鲢群体中有变异的基因座位为GPI*;津鲢与荆州鲢的多态基因座位(最高基因频率≤0.99)比例和平均杂合度预期值分别为11.11%、5.56%和0.0150、0.0011,群体间Nei遗传距离为0.00059;2群体GPI*基因座位最高基因频率间的χ2检验结果表明这2个群体间呈极显著差异。[结论]为津鲢的大面积推广奠定理论依据。
基金
天津市细胞遗传与分子调控重点实验室开放研究基金资助项目
天津市科技支撑计划项目(09zckfnc02100)~~
