摘要
为了研究蜂王浆酶解产物在不同湿度环境下的吸湿保湿性能,利用碱性蛋白酶对蜂王浆进行酶解,以蜂王浆冻干粉和骨胶原为对照,对蜂王浆酶解产物在25℃,相对湿度分别为20%、50%和65%条件下酶解产物的吸湿和保湿性能进行测定。结果表明:在本实验湿度环境范围内,在底物质量分数为8%、酶与底物含量比1%、酶解温度45℃、酶解时间3h、pH值9.5的酶解条件下,酶解产物的吸湿和保湿性能均高于蜂王浆冻干粉,且明显优于骨胶原。说明蜂王浆通过碱性蛋白酶酶解的产物具有良好的保湿和吸湿性能,在吸湿保湿化妆品开发方面具有广阔的开发前景。
In order to research the moisture adsorption and retention capacity of enzymatic hydrolysates from royal jelly under different humidity conditions, the royal jelly proteins were hydrolyzed by alkali protease, and the moisture adsorption and retention capacity of their hydrolysates were determined under the conditions of 25℃, relative humidities of 20%, 50% and 65%, respectively, in comparison with lyophilized royal jelly powder and bone collagen. The results showed that the enzymatic hydrolysates exhibited better performance both in moisture adsorption and retention test than lyophilized royal jelly powder and hone collagen did. The optimum conditions were as follows： substrate concentration of 8%, enzymatic concentration of 1%, temperature of 45℃, enzymatic hydrolysis time of 3 h and pH 9.5. It is safe to draw the conclusion that enzymatic hydrolysis will he an efficient method for ohtaining ingredients from royal jelly with good moisture adsorption and retention capacity and it has a bright future in exploitation of moisture-keeping or moisture-adsorbing cosmetic.
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2012年第4期504-510,共7页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家星火计划"蜂产业共性关键技术研究与集成应用"资助项目(2010GA701004)
浙江省新苗人才计划资助项目(2010R441001)
关键词
蜂王浆
碱性蛋白酶
水解
吸湿
保湿
royal jelly
alkali protease
hydrolysis
moisture adsorption
moisture retention
