摘要
超低温保存是目前木本植物长期保存的理想方法。综述国内外超低温保存技术在木本植物种质资源保存方面的应用现状和超低温条件下的低温伤害及细胞超微结构改变,阐述超低温保存技术的发展及各类超低温保存技术的优缺点和影响因素,指出广谱适用性问题、保存方法的可重复性问题和可操作性问题、投入产出问题是影响超低温保存技术推广应用的主要问题,认为扩大超低温保存木本植物资源的种类、根据植物种类和外植体类型发展相应的超低温保存技术、根据保存目的合理选取保存材料及其保存方法、优先发展经济优势物种的保存技术、增加财政投入以研发新型超低温保存技术是解决上述问题的重要途径。
Cryopreservation is considered to be a valuable solution for long-term storage of plant germplasm especially for woody plant species. In this article, we summarized the application of cryopreservation in woody plant species, the damage and ultrastructural changes of freezing cell and the development of various cryopreservation techniques and their advantages and disadvantages. We pointed out that the spectrum, repeatability and maneuverability of cryopreservation plus high investment and low output of this technique were main problems existed at present. To solve these problems, various suggestions were put forward, such as expanding the woody plant resources for cryopreservation, developing cryopreservation techniques according to plant species, choosing the right conservation materials and methods based on the purpose of preservation, giving priority to development of preservation techniques for economic species and raising investment for developing new cryopreservation methods.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第4期347-353,共7页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家教育部项目(IRT0963)
关键词
木本植物
种质资源
超低温保存
低温伤害
woody plant species
germplasm resources
cryopreservation
low-temperature damage
