摘要
猕猴桃茎段产生的愈伤组织，经过冷驯化处理后，加入５％ＤＭＳＯ和１０％葡萄糖，以一１℃·ｍｉｎ^(-１)降温速率降至一６０一－７５℃，投入液氮中保存。在液氮中存放时间的长短对愈伤组织存活率没有影响，在３５一４０℃水浴中迅速化冻，将化冻后的材料接种在ＭＳ附加０．０５ｍｇ·１^(-１)２，４一Ｄ及１．０ｍｇ·１^(-１)ＺＴ的培养基上暗培养，待开始恢复生长转在光下培养１５一２０ｄ，再转接在只含有１．０ｍｇ·１^(-１)ＺＴ的ＭＳ分化培养基上，２０一３０ｄ后分化出芽，并形成植株。
Kiwifruit(Actinidia deliciosa)calli occured on MS media after cooling acclimatization
were put into cry-opreservation agent composed of 5%DMSO and 10%glucose,by step-freezing
with￣-1℃· min￣(-1) to 60℃一-75℃ and immersed in liquid nitrogen(LN2).The survival
rates of cells had no significant differ-ence during storage inLN2.The calli were thawed in water
bath at 35℃一40 ℃ and cultured on MS mediawith 1mg·l￣(-1)ZT plus 0.05 1mg·1￣(-1)2,4
一Din dark,when calli resumed to grow,the samples were movedinto light condition for 15一20
days,they would differentiate meristem and induce shoots on MS media
with1mg·1-1ZT
出处
《果树科学》
CSCD
北大核心
1996年第2期88-91,共4页
Journal of Fruit Science
基金
高等学校博士学科点专项科研基金
关键词
猕猴桃
愈伤组织
超低温保存
Kiwifruit(Actinidia deliciosa)
Callus
Cryopreservation
