摘要
目的 探讨血管紧张素转换酶 (ACE)基因多态性与冠心病危险因素的关系。方法 应用聚合酶链反应技术和遗传学方法 ,测定 15 9名汉族正常人、148例冠心病患者的ACE基因插入 /缺失 (I/D)多态性频率 ,并调查冠心病患者经典危险因素。结果 ①ACE基因型分布与冠心病患者的年龄、性别、体重指数 (BMI)、收缩压、舒张压、总胆固醇 (TC)、甘油三酯 (TG)、低密度脂蛋白(LDL)、高密度脂蛋白 (HDL)、HDL2 、HDL3 、载脂蛋白 (Apo)AI、ApoB、脂蛋白〔Lp(a)〕以及高血压病(HT)、吸烟、糖尿病 (DM )和肥胖间差异均无显著性 (P均 >0 .0 5 ) ;②以ApoB <12 5mg/dl和BMI≤2 4kg/m2 ;或TC <2 30mg/dl、TG <2 0 0mg/dl、LDL <16 0mg/dl和BMI≤ 2 4kg/m2 ;及不合并HT和DM者定义为冠心病低危险亚组 ,分别与正常人比较 ,DD基因型频率 (构成比 )分别为 0 .2 5 7、0 .315和0 .2 73,均显著高于正常人 (χ2 =3.98、7.80、4.0 1,P均 <0 .0 5 )。结论 ACE基因多态性与冠心病经典危险因素无关 ,DD基因型可能是中国人冠心病的一个独立危险因素。
Objective To explore the relationship between angiotensin converting enzyme(ACE)gene polymorphism and risk factors for coronary heart disease(CHD). Methods 148 patients with CHD and 159 unrelated healthy individuals were studied using polymorase chain reaction (PCR) and genetic methods. Risk factors of CHD were assessed by standard questionnaire, physical examination and blood tests. Results ①There were no significant differences among genotypes in terms of years, sex, body mass index(BMI),systolic blood pressure, diastolic blood pressure, total cholesterol(TC), triglyceride(TG), low density lipoprotein cholesterol(LDL), high density lipoprotein cholesterol(HDL), HDL 2, HDL 3, apolipoprotein(Apo)AI, ApoB, and lipoprotein (a)〔Lp (a)〕. The prevalence rates of hyperlipidemia, hypertension(HT), cigarette smoking, diabetes mellitus(DM) and obesity were similar in the ACE genotypes ( P > 0.05 ). ② The DD genotype frequencies in low CHD risk groups, as defined by ApoB<125 mg/dl and BMI≤24 kg/m 2, or TC<230 mg/dl, TG<200 mg/dl, LDL<160 mg/dl and BMI ≤24 kg/m 2 without HT and DM were significantly higher than that in normal subjects ( 0.257 , 0.315 , 0.273 vs 0.157 ,χ 2= 3.98 、 7.80 、 4.01 , P < 0.05 ). Conclusion There was no correlations between ACE gene polymorphism and the classic CHD risk factors noticed. ACE DD genotype seemed to be an independent risk factor for CHD in the Chinese population.
出处
《中华流行病学杂志》
CAS
CSCD
北大核心
2000年第3期208-211,共4页
Chinese Journal of Epidemiology
基金
浙江省医药卫生科研基金!资助 (编号 :962 1 5)
