摘要
目的观察单核细胞趋化因子(MCP-1)、基质金属蛋白酶-9(MMP-9)、肿瘤坏死因子(TNF-α)和胰岛素样生长因子(IGF-1)在人主动脉粥样硬化组织中表达的情况。方法病变组主动脉组织取自外科手术腹主动脉瘤患者,对照组主动脉组织取自非正常死亡健康成人尸体的正常主动脉。用免疫组化方法检测MCP-1,MMP-9,TNF-α和IGF-1在动脉粥样硬化斑块处的表达及定位。结果病变组MCP-1、TNF-α、MMP-9、IGF-1的表达水平显著高于对照组,差异均有统计学意义(P均<0.05)。MCP-1蛋白表达与MMP-9(rs=0.464,P<0.05)、TNF-α表达呈正相关(rs=0.516,P<0.05),但与IGF-1表达无相关性(rs=0.370,P>0.05)。结论 MCP-1、MMP-9、TNF-α和IGF-1在人动脉粥样硬化中高表达,MCP-1对MMP-9、TNF-α有促进的作用。
Objective To observe the expressions of monocyte chemoattractant protein-1 （MCP-1）, matrix metalloproteinase-9 （MMP-9） , tumor necrosis factor-or （TNF-α） and insulin-like growth factor-1 （IGF-1） in human atherosclerotic tissue. Methods The atherosclerotic samples of disease group were colleted from the patients with ab- dominal aneurysm after surgery, and normal aorta samples of control group were collected from health adults died from accidents. The expressions and distributions of MCP-1, MMP-9, TNF-α and IGF-1 in atherosclerotic plaques were de- tected by using immunohistochemistry technique. Results The expressions of MCP-1, MMP-9, TNF-α and IGF-1 were significantly higher in disease group than those in control group （ all P 〈 0.05 ）. The expression of MCP-1 was positive- ly correlated to MMP-9 （ rs =0.464,P 〈0.05） and TNF-α （re =0.516 ,P 〈0.05） ,but was not correlated to IGF-1 （ rs = 0. 370,P 〉 0.05 ）. Conclusion The expressions of MCP-1, MMP-9,TNF-α and IGF-1 are higher in human athero- sclerotic tissue,and MCP-1 has an improvement effect on MMP-9 and TNF-α .
出处
《中国循证心血管医学杂志》
2012年第3期215-217,共3页
Chinese Journal of Evidence-Based Cardiovascular Medicine
