摘要
目的:了解人博卡病毒(human bocavirus,HBoV)在南京地区呼吸道感染患儿中的致病情况。方法:选取2009年11月～2010年6月因呼吸道感染住院的患儿鼻咽吸取物标本共100份,采用聚合酶链反应(PCR)方法检测HBoV的NP1基因片段,并对阳性产物进行测序,利用多功能分析软件DNAstar进行同源性分析,MEGA软件绘制遗传进化树。分析感染患儿的临床资料。结果:在100例鼻咽吸取物标本中共检测到5例阳性,阳性率为5%。DNAStar分析发现5例阳性标本之间核苷酸同源性达99.7%～100.0%,与其他国家HBoV的株同源性为99.4%～100.0%。临床资料显示这5例患儿临床症状主要表现为咳嗽、喘息、发热等,临床诊断为肺炎2例、毛细支气管炎1例、支气管炎1例及喘息性支气管炎1例。患儿经常规治疗后均痊愈出院。结论:南京地区呼吸道感染患儿中有HBoV的流行。
Objective：To understand whether human bocavirus（HBoV） is one of the pathogens leading to the children’s respiratory infections in Nanjing.Methods：The patients hospitalized for respiratory tract infections（RTIs） were enrolled in the present study.Nasopharyngeal aspirates（NPAs） were collected from November 2009 to June 2010.HBoV NP1 gene in the samples was determined by polymerase chain reaction（PCR）.The amplified positive NP1 fragments were sequenced and alignment with other HBoVs by DNAStar.Phylogenetic trees were drawn with MEGA software.The clinic findings of the children with HBoV infection were collected and analyzed.Results：Of all the 100 NPAs,five positive ones were founded.Among the five positive samples,the sequence homogeneity of the amplified NP1 gene fragment were 99.7%～100.0% at the nucleotide level.As compared with HBoVs from other countries,the homogeneity were 99.4%～100.0%.Among the five patients,the diagnoses were bronchopneumonia,bronchiolitis and bronchitis.Clinic findings included fever,cough,wheezing,etc.The patients were all recovered with conventional therapy.Conclusion：HBoV was one of the pathogens leading to the children’s respiratory tract infection in Nanjing.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2012年第8期1099-1102,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学校科技发展基金(09NJ-MUM043)
关键词
人博卡病毒
呼吸道感染
儿童
human bocavirus； respiratory infection； children
