摘要
在－１５０～＋２０℃范围测定了藻红蓝蛋白α－亚基的低温紫外－可见吸收光谱和圆二色光谱．通过这些光谱测定表明：在温度低于－９０℃时藻红蓝蛋白α－亚基可逆光化学反应很小，在温度高于－７０℃时藻红蓝蛋白α－亚基能发生可逆光化学反应但不存在中间态．－７０～－９０℃时藻红蓝蛋白α－亚基的可逆光化学反应在紫外－可见吸收光谱和圆二色光谱中产生特定的信号，它们可能相应于藻红蓝蛋白α－亚基可逆光化学反应过程的中间态．这些实验还表明：藻红蓝蛋白α－亚基的脱辅基蛋白没有形成一个特定的空间来允许其辅基色素发生可逆光化学顺反异构化。
The reversible photochemistry of phycoerythrocyanin α subunit (α PEC) has been measured by low temperature absorption and circular dichroism from -150℃ to +20℃. It is shown that a) lower than -90℃, the photochemistry is silent; b) higher than -70℃, the photochemistry increases gradually and no indication for intermediate; c) from -70℃ to -90℃, some signals in absorption and circular dichroism can be observed that maybe represent certain intermediates on reversible photochemistry of α PEC. The results suggest that in α PEC there does not exist sufficient space for isomerization of chromophore, and the isomerization of chromophore must then be assisted and facilitated by concomitant structural changes of apoprotein.
出处
《武汉大学学报（自然科学版）》
CSCD
1999年第2期207-210,共4页
Journal of Wuhan University(Natural Science Edition)
基金
国家自然科学基金
德国科学研究联合会基金
德国亚历山大.冯.洪堡基金
关键词
藻红蓝蛋白
α-亚基
圆二色光谱
吸收光谱
phycoerythrocyanin α subunit
low temperature absorption spectroscopy
low temperatur circular dichroism spectroscopy
