摘要
光子计数成像系统可以探测生物超微弱发光 ,但是只能探测生物的宏观图像 ,若要深入到细胞、分子水平 ,必须有显微光子计数成像系统。二者的区别在于显微光子计数成像系统是噪声受限系统。本文报道的显微光子计数成像系统 ,采用14 C同位素光源来监测系统的状态 ,保证实现极限探测。该系统可以用来研究痕量生物分子的分布和功能 ,显示钙离子在细胞内外的分布 ,活性氧、基因表达的监测等。由单光子到单分子、组织学图像到功能图像的转变 ,将是重要的发展 ,为生物学医学的应用提供了光明的前景。
The photon counting image system is able to detect the ultra weak bioluminescence of the macro image of organisms. To study the luminescence of cell or molecules, the microscopic photon counting image system is necessary. The difference of two systems is that the microscopic photon counting image system is a noise limited system. The light source of radioisotope 14 C is used for examination the state to ensure the limit detect of microscopic photon counting image system. The fundamental capability of the system is to measure the distribution and function of extremely small amounts of biomolecules, to realise real time visualisation of oxyradical burst activities, continuous observation of the calcium ion wave moving, and monitoring of gene expression etc. It will be an important development of transform from photon to molecule and from image of histology to image of function, promising for applications in the field of biology and medical science.
出处
《光学学报》
EI
CAS
CSCD
北大核心
2000年第8期1072-1076,共5页
Acta Optica Sinica
基金
国家自然科学基金资助项目
关键词
显微光子计数成像系统
生物超微弱发光
microscopie photon counting image, radioisotope 14 C , active oxygen.
