摘要
目的 探讨脂多糖诱发急性肺炎时 ,大白鼠中性粒细胞碱性磷酸酶 (alkaline phosphatase,AL P)的超微定位及其在炎症过程中的相关功能。 方法 采用腹腔内 (intraperitoneal,IP)注射及气管内 (intratracheal,IT)滴注脂多糖 (lipopolysaccharide,L PS,一种提自大肠杆菌的内毒素 )的方式诱发大白鼠急性肺炎 ,使中性粒细胞在肺内不同部位集聚。用铈作为捕捉剂 ,以检测 AL P活性。对照组为介质中除去底物或加入 2 m mol/L左旋咪唑。电镜下行酶活性定位观察。 结果 IP注射主要导致中性粒细胞在鼠肺肺泡隔毛细血管内集聚 ,而 IT注射多致中性粒细胞聚集于肺泡腔中。在偶见的非炎区肺内中性粒细胞 ,AL P活性产物主要以小球状或管状的膜包颗粒存在于胞质中。L PS腹腔内注射组中 ,AL P活性除出现于胞质内 ,还呈现于中性粒细胞的质膜表面 ;在气管内给药组 ,与毛细血管内皮细胞或肺泡上皮细胞相接触的中性粒细胞质膜表面 AL P活性有所增强 ,而肺泡腔的中性粒细胞质膜表面 AL P活性减弱。在各组标本中 ,对照组均为 AL P阴性。 结论 大鼠肺中性粒细胞 AL P活性可经 L PS注射而增强 ,其活性可能和细胞与细胞间的粘连、细胞外环境以及与细胞的功能状态有关。
Objective\ To explore the cytochemical localization of alkaline phosphatase(ALP) in neutrophils in the rat lung and the related function following the LPS\|induced acute pneumonia. Methods\ The accumulations of the neutrophils in the different parts of the lung were induced by intraperitoneal(IP) or intratracheal(IT) injection of lipopolysaccharide(LPS, a kind of endotoxin from Escherichia coli).The detection of ALP activity was performed in a cerium\|based reaction medium.The controls included a substrate\|free medium or a medium containing 2mmol/L levamisole.The localization of the ALP activity was observed by a electron microscope. Results\ The IP\|injection of LPS induced mainly a significant accumulation of the neutrophils within the capillaries of alveolar septum,while the IT\|injection of LPS resulted in a high accumulation in the alveolar spaces mainly.The ALP activity was localized predominantly in small spherical and tubular membrane\|bounded granules in neutrophils and observed occasionally in the non\|inflamed regions of the rat lung.Following the IP\|injected.Prominent ALP activity was found both on the plasma membrane surface and the cytoplasm of the neutrophils.In the IT\|injected rat,the ALP activity was significantly increased on the membrane surface of the neutrophils contacting to the endothelial cells of the capillaries or the alveolar epithelial cells in the pneumonia region,while showed a weak reaction on the surface of the neutrophils which suspended in the alveolar spaces.No enzyme activity was detected at the endothelial plasma membranes in all of the samples.Negative reactions were showed in the controls. Conclusion\ ALP activity of neutrophils in the rat lung can be increased by LPS stimulation and its activity may be related to cell\|cell interaction,the cell surroundings and the different functional status of the neutrophils.
出处
《解剖学报》
CAS
CSCD
北大核心
2000年第1期52-55,I009,共5页
Acta Anatomica Sinica
基金
教育部留学回国人员科研启动基金!(教外司留 [1997] 83 2号 )
日本文部省科研文化基金
关键词
中性粒细胞
碱性磷酸酶
脂多糖
肺炎
Cytochemistry
Neutrophil
Alkaline phosphatase
Lipopolysaccharide
Rat lung.
