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培养心肌细胞缺血性损伤的细胞化学定量研究 被引量:3

THE QUANTITATIVE STUDY OF CELLULAR CHEMISTRY IN ISCHEMIC INJURY BY CULTURED HEART CELLS
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摘要 本文利用图象分析系统(Texture Analysis System TAS)动态观察了缺血早期心肌细胞内乳酸脱氢酶(LDH)、琥珀酸脱氢酶(SDH)及糖原(PAS)的变化规律,TAS 的定量细胞化学表明,缺血早期LDH 活性呈双相改变,缺血60分钟LDH 活性有所增高,至120分钟明显下降;但SDH 活性在缺血60分钟已明显降低,SDH 活性下降早于并重于LDH 活性的改变. The alleration patterns of tactate dehydrogenase(LDH),succinate dehydrogenase(SDH)and glycogen(positive substance of periodic acid/Schitt reaction,PAS)in cultured heart cells were observed by means ofTexture Analysis System(TAS)in the early stage ofischemia.The results of the quantitative cellular chemistryindicate that the change of LDH activity is of biphase.LDH activity has a little increase at 60 min followingischemia but decrease rema-kably at 120 min.However,the decrease of SDH activity is detectable at 60 min fol-lowing ischemia and the change is earlier and more obvi-ous than that of LDH activity.
出处 《西安医科大学学报》 CSCD 1989年第2期110-112,共3页 Journal of Xi'an Medical University(Chinese)
关键词 心肌细胞 培养 缺血 细胞化学 cultured heart cell ischemia quantitative cellular chemistry
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参考文献1

  • 1Daniel Acosta,Marianne Puckett,Reagan McMillin. Ischemic myocardial injury in cultured heart cells: Leakage of cytoplasmic enzymes from injured cells[J] 1978,In Vitro(8):728~732

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