摘要
目的探讨重组人白介素-10(IL-10)对急性坏死性胰腺炎(ANP)胰腺组织病理改变的影响,为临床治疗ANP提供新的途径及理论依据。方法健康雄性SD大鼠92只,随机分成正常对照组(C组)24只、ANP组(A组)36只,IL-10干预组(I组)32只。A组大鼠分3次给予腹腔内注射6%的左旋精氨酸(L-Arginine),诱导ANP;I组大鼠于腹腔内注射L-Arginine诱导后2 h、5 h、8 h分别给予腹腔内注射重组人IL-10 1万U,共3万U;C组按A组时点给予0.9%生理盐水腹腔内注射作正常对照。在注射诱导ANP后4 h、12 h、24 h、36 h时分批处死大鼠,对大鼠胰腺组织进行组织病理学评分。结果 A组大鼠各时点胰腺组织病理学评分明显高于C组(P<0.01),以36 h时为最高;I组除4 h时外,其余各时点胰腺组织病理学评分均低于A组(P<0.05);I组各时点血清淀粉酶水平均低于A组(P<0.05)。结论早期应用重组人IL-10可以降低ANP炎症反应程度,减轻病理损害,有可能成为治疗ANP的新途径。
Objective To investigate the effects of recombinant human interleukin-10（IL-10） on histopathological changes in rats with acute necrotizing pancreatitis（ANP）. Methods Ninety-two male SD rats were randomly divided into three groups：normal control group（ Group C ,n = 24）, ANP group （Group A,n = 36） and IL-10 interference group （Group Ⅰ, n =32）. Group A received three intraperitoneal injection of 6% L-arginine（3×1.0 mg/g） at hourly intervals. Group Ⅰ was treated with 10 000 unites of intmperitioneal recombinant human IL-10 at the 2nd,5th and 8th hour after the last injection of barginine. Group C received three intraperitoneal injection of 0.9% saline alone at hourly intervals three times. Rats were killed at the 4th,12th,24th and 36th hour after the last L-arginine injection. Histopathological scores were recorded according to the edema,inflammation and necrosis of pancreatic tissues in three groups. Results Pancreatic histopathological scores in Group A were higher than those in Group C at the 4th,12th,24th,36th hour after the last L-arginine injection（P 〈0.01） ,the highest was at the 36th hour;Pancreatic histopathological scores in Group Ⅰ were lower than those in Group A at the 12th,24th,36th hour （P 〈0. 05） ;The serum amylase level at each time point in Group Ⅰ were lower than those in Group A （P 〈0. 05）. Conclusion Early application of recombinant human IL-10 can attenuate ANP inflammatory response and relieve the histopathological injury, which may be a new therapy for acute necrotizing pancreatifis.
出处
《广西医学》
CAS
2012年第7期816-818,共3页
Guangxi Medical Journal
基金
广西自然科学基金(桂科自0449054)
关键词
急性坏死性胰腺炎
白介素-10
病理改变
Acute necrotizing pancreatitis
Interleukin-10
Histopathological change
