摘要
目的:探讨重组人白介素10(IL-10)对急性坏死性胰腺炎(ANP)血清IL-6水平的影响,为临床治疗ANP提供新的途径及理论依据。方法:健康雄性SD大鼠92只,随机分成正常对照组(C组)、ANP组(A组)和IL-10干预组(I组)。A组大鼠分3次给予腹腔内注射6%的左旋精氨酸(L-Arginine)3×1.0mg/g体重,每次间隔1h,诱导ANP;I组于L-Arginine注射诱导胰腺炎后2,5,8h分别腹腔内注射重组人IL-10 1万U,共3万U;C组大鼠按上述时点给予0.9%生理盐水腹腔内注射作正常对照。在注射诱导胰腺炎后第4,12,24,36小时共4个时点分批处死大鼠,对胰腺组织进行组织病理学评分,并用酶联免疫吸附法(ELISA)检测血清淀粉酶水平、血清IL-6水平。结果:A组各时点IL-6、血清淀粉酶水平以及胰腺组织病理学评分较C组明显增高(P<0.05或P<0.01);其中,胰腺组织病理学评分及血清淀粉酶升高以第24,36小时最为显著;IL-6在4h达高峰;I组组织病理学评分(除第4小时外)、血清淀粉酶及IL-6各时点值均显著低于A组(P<0.05或P<0.01)。结论:早期应用重组人IL-10可以抑制炎症细胞因子IL-6释放,降低炎症反应的严重程度,减轻实验性胰腺炎的病理损害。
Objective： To investigate the effects of recombinant human interleukin （IL） 10 on serum interleukin-6 levels in L-arginine-induced acute necrotizing pancreatitis rats. Methods：Ninty-two Sprague-Dawley rats were randomly divided into three groups. Group A （ n =36） and Group I （ n =32） received three intraperitoneal injection of 6% L arginine （3 × 1.0 mg/g） at hourly intervals. Group I was also treated with 10 000 unites of intraperitioneal recombinant human IL 10 at the 2nd, 5th and 8th hours after the last injection of L-arginine. Group C （ n =24） received saline alone. Rats were killed at the 4th, 12th, 24th and 36th hours after the last L-arginine injection. Serum interleukin-1β and amylase were assayed. Pancreatic tissues were fixed in formalin. Histopathological score was recorded according to the edema, inflammation and necrosis of the tissues in three groups. Results： Compared with group C, serum amylase and Interleukin-6 ,pancreatic histopathological scores in group A increased significantly after L-arginine injection （ P 〈 0.05 or P〈0.01）. Compared with group A , the levels of serum amylase,Interleukin-6 in group I were significantly decreased at various time points （ P 〈0.05 or P 〈0.01）. Pancreatic histopathological scores in group I were decreased as compared with group A at the 12th, 24th, 36th hours （P 〈0.05 or P 〈 0.01）. Conclusion： IL-10 attenuates the severity of experimental acute necrotizing pancreatitis by inhibiting the release of interleukin-6.
出处
《广西医科大学学报》
CAS
2012年第4期507-509,共3页
Journal of Guangxi Medical University
基金
广西自然科学基金资助项目(桂科自0447054)
