摘要
以水稻内源基因SPS、外源抗虫基因Cry1Ab、外源抗虫基因Cry1Ab/Ac、外源抗虫基因Btc、报告基因GUS、NOS终止子和CaMV35S启动子为检测对象,设计7对引物,通过优化PCR扩增体系中不同引物浓度的配比及退火温度,建立水稻转基因成分的七重PCR检测体系。结果表明:建立的七重PCR体系能有效检测出水稻及其他作物(大豆、玉米、棉花籽、菜籽粕)中的转基因成分,检测过程简便、特异性好。
In order to detect transgenic components in genetically modified rice, endogenous gene SPS, exogenous insect resistant gene CrylAb/Ac, exogenous insect resistant gene CrylAb, exogenous insect resistant gene Btc, reporter gene GUS, NOS terminator and CaMV35S promoter were evaluated by multiplex polymerase chain reaction. Seven pairs of primers were designed, and primer concentration and annealing temperature were optimized to establish a multiplex PCR system. The results showed that the developed seven-plex PCR system could allow the detection of transgenic components in genetically modified rice and other crops including soybean, corn, cottonseeds and rapeseed meal with high efficiency and good stability.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2012年第12期159-162,共4页
Food Science
基金
农业部转基因重大专项(2008ZX08012-005)
