摘要
目的 :研究胚胎脊髓移植联合应用神经生长因子 (NGF)及尼莫地平 (ND)对损伤脊髓的修复作用。方法 :90只Wistar大鼠半切洞脊髓损伤后 ,随机分为 3组 :(1)单纯胚胎脊髓移植组 (A组 ) ,(2 )移植 +NGF组 (B组 ) ,(3)移植 +NGF +ND组 (C组 )。观察组织形态学、运动诱发电位及行为学变化。结果 :C组的运动诱发电位潜伏期在早期比A组、B组皆有明显缩短 (P <0 0 5 )。以后肢运动功能的Tarlov评分看 ,B组和C组的后肢运动功能明显优于A组 (P <0 0 5 )。病理观察显示 ,移植的胚胎脊髓可在宿主内分化、发育 ,并可挽救损伤神经元 ,同时可与宿主脊髓结构连接。结论 :胚胎脊髓移植与NGF、ND合用 ,可能是脊髓损伤的一种有效疗法。
Objective:To study the effect of fetal spinal cord grafts,Nerve growth factor(NGF) and Nimodipine(ND) on repairing injured spinal cord.Method:90 rats were hemisection cavity injured at the spinal cord,then divided into transplantation group(A),Transplantation+NGF treated group(B),Transplantation+NGF+ND treated group(C).Motor evoked potentials(MEP),motor function of hindlimbs(Tarlov′s Score) and pathological changes were observed.Results:Tarlov′s score in B and C groups were higher than that in A group(P<0 05),NGF and ND could shorten the latencies of MEP.The pathological observation indicated that fetal spinal cord(FSC) could grow and differentiate in the host spinal cord and rescue the injured neurons,and the fiber connections between FSC and host spinal cord were reconstructed.Conclusions:The combined application of FSC transplantation,NGF and ND may be an effective therapy for spinal cord injury. Author′address Research institute of surgery,Third Military Medical University,Chongqing,400042
出处
《中国脊柱脊髓杂志》
CAS
CSCD
2000年第3期151-153,共3页
Chinese Journal of Spine and Spinal Cord
基金
全军医药卫生科研基金资助!( 96Z0 13 )
关键词
胚胎组织移植
神经生长因子
尼莫地平
脊髓损伤
Fetal tissue transplantation
Nerve growth factor
Nimodipine
Spinal cord injury
