摘要
目的了解子宫内膜组织中MicroRNA-29b的表达,探讨其在子宫内膜癌变中的意义。方法选取甲醛固定石蜡包埋组织96例,其中正常子宫内膜16例,不典型性增生内膜20例,子宫内膜癌60例。设计Mi-croRNA-29b和内参U6的茎环结构反转录引物和PCR扩增引物,利用实时荧光定量PCR技术检测各种子宫内膜组织中MicroRNA-29b的含量,分析MicroRNA-29b表达与子宫内膜癌临床病理特征及雌、孕激素受体(ER、PR)的关系。结果子宫内膜癌组织中MicroRNA-29b的表达低于正常子宫内膜、不典型性增生内膜组织,差异有显著性(F=2.998、3.690,t=3.163、2.297,P<0.05)。子宫内膜癌组织中MicroRNA-29b的表达与FIGO分期及淋巴结转移有关(t=-2.070、-2.773,P<0.05)。ER、PR表达阳性和阴性子宫内膜癌组织MicroRNA-29b表达比较,差异无显著性(P>0.05)。结论 MicroRNA-29b的表达失调可能与子宫内膜癌的发生、发展有关,对子宫内膜癌的早期诊断、预后评估可能有一定的指导意义。
Objective To detect the expression of MieroRNA 29b in tissue of endometrium, and explore its significance during caneeration of endometrium. Methods Ninety-six formalin-fixed paraffin-embedded tissue blocks were collected in this study, of which, 16 were normalendometrium, 20 were atypical hyperplasia, and 60 were endometrial cancer （EC）. According to the sequence of MieroRNA-29b and U6, the stem-loop reverse transcriptional primers and the polymerase chain reaction primers were designed, the expression of MicroRNA-29b in endometrium was detected by quantitative real time polymerase chain reaction, the association of the MicroRNA-29b expression with clinicopathologie features of EC, estrogen receptor （ER）, and progesterone reeeptor（PR） were analyzed. Results The expression of MicroRNA-29b in EC was lower than that in normal endometrium and atypical hyperplastic endometrium （F= 2. 998,3. 690;t= 3. 163,2. 297 ;P〈0.05）. The expression of MieroRNA-29b in EC was as- sociated with FIGO staging and lymph node metastasis （t=2. 070, -2. 773 ;P〈0.05）. The differences between negative ER and PR expression and MicroRNA-29b in EC were not significant （P〉0.05）. Conclusion The expression disturbance of MieroR- NA-29b is probably associated with the occurrence and development of endometrial cancer, which may he helpful for early diagnosis and predicting the prognosis of this malignaney.
出处
《齐鲁医学杂志》
2012年第5期389-391,394,共4页
Medical Journal of Qilu
关键词
微RNAS
子宫内膜肿瘤
聚合酶链反应
受体
雌激素
受体
孕激素
MicroRNAs
endometrial neoplasm
polymerase chain reaction
receptors, estrogen
receptors, progester-one
