摘要
目的：探讨人乳头瘤病毒（ＨＰＶ）１６、１８型感染与人乳腺癌病因学的关系。方法：采用免疫组化法（ＳＰ）检测 ＨＰＶ １６、１８ Ｅ６蛋白在 １０例正常乳腺组织，４５例乳腺癌组织中的表达并对癌组中 １３例 ＨＰＶ １６、１８ Ｅ６蛋白阳性材料进行 ＨＰＶ １６、１８ ＤＮＡ原位杂交检测。结果：癌组中 ＨＰＶ １６、１８ Ｅ６阳性率为 ５３．３％（２４／４５），而正常乳腺组织中均为阴性表达。 ＨＰＶ １６、１８ ＤＮＡ阳性率为 ３８．５％（ ５／１３）。结论：高危型 ＨＰＶ １６、１８感染可能涉及乳腺癌的发生过程。
Objective: To found the etiologic relationship between human papillomavirus (HPV) types 16 and 18 infection and human breast cancer. Methods: Using streptavidin/peroxidase (SP) immunohistochemical techniqueic cases of normal breast tissues and 45 cases of breast cancers were studied on the expression of HPV 16 and 18 E6 protein, and 13 cases of HPV 16 and 18 E6 protein positive were studied by in situ hybridization using HPV 16 and18 DNA probes in cancer group. Results: HPV 16 and 18 E6 protein positive rate was 53.3% (24/45 ) in cancer group, and HPV 16 and 18 DNA positive rate was 38.5% (5/13 ) in 13 cases positively expressing HPV 16 and 18 E6 proteins, but all of 10 cases of the normal breast tissues showed negative expression in HPV 16 and 18 E6 protein. Conclusion: HPV 16 and 18 infection may be involved in the carcinogenesis of breast cancer.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第1期48-50,共3页
Chinese Journal of Cancer
基金
广西自然科学基金!(9912043)
关键词
乳腺癌
人乳头瘤病毒
原位杂交
Breast neoplasma
Human papillomavirus
Immunohistochemistry
In situ hybridization
