摘要
采用淋巴细胞毒试验、淋巴细胞转化试验及吞噬细胞功能实验等多项细胞免疫学指标测定 ,以及组织形态学观察与生物力学测定 ,比较深低温冷冻与深低温冷冻干燥两种不同处理方式对同种异体肌腱移植的差异 ,并综合评价各种移植的效果。结果显示 :①深低温冷冻移植组及深低温冷冻干燥移植组的淋巴细胞死亡率、转化率及吞噬细胞吞噬率均低于未经处理的同种异体移植组 (P <0 .0 5) ,而与自体肌腱移植组差异无显著性 (P >0 .0 5) ;②深低温冷冻移植组、深低温冷冻干燥移植组及自体肌腱移植组肌腱无明显坏死 ,但腱周出现轻度粘连和结合部膨大 ,未经处理的同种异体肌腱移植组肌腱变性坏死 ;③光镜、电镜下未经处理的异体肌腱移植组肌腱腱束变性坏死 ,而深低温冷冻移植组与冷冻干燥移植组则无明显差别。上述结果表明 ,深低温冷冻干燥或冷冻但未经干燥的同种异体肌腱在免疫学、生物力学、形态学方面的表现与自体肌腱移植结果基本相同 ,而未经处理的异体肌腱因免疫排斥反应大 。
A series of immunologic tests, including lymphocyte cell toxicity test, as well as lymphocyte transformation test, macrophage function test, histological examination, and biomechanical measurement were carried out in guinea pigs to compare the effect of deep freezing(Group Ⅰ) with deep freezedrying(Group Ⅱ) on tendon allografts. The results revealed that the lymphocyte mortality, lymphocytic transformed rate and phagocytizing rate of the macrophages in Group Ⅰ and Group Ⅱ were lower than those in non processed allograft group(Group Ⅲ) and had no statistical difference among Group Ⅰ, Group Ⅱ and Group Ⅲ. As compared with the non processed allograft, the freezed and freeze dried allografts showed ameliorated immunologic reactions, mild morphological lesion and better mechanical strength after transplantation. The results suggest that deep freezing and deep freeze drying processings are valid measures in tendon allograft treatment. [
出处
《湖南医科大学学报》
CSCD
2000年第2期138-140,共3页
Bulletin of Hunan Medical University
关键词
肌腱移植
同种异体移植
豚鼠
transplantation,homologous,heterologous
freeze drying *
tendon *
musculoskeletal system
guinea pigs
