摘要
以啤酒糟为主要原料,采用黑曲霉3.316(Aspergillus niger 3.316)固态发酵生产纤维素酶,对培养基组成和培养条件进行优化。结果表明:啤酒糟和麸皮的质量比为8∶2、水料质量比为1.5∶1、硫酸铵质量分数为3%、发酵时间为4d时,所产纤维素酶活性最大,滤纸酶活性(FPA)和羧甲基纤维素酶活性(CMCA)分别达72.611 8和692.1700nkat。
Cellulase production was carried out by Solid-State Fermentation （SSF） with Aspergillus niger 3. 316 using brewer＇s spent grain as the main material. The SSF con- ditions were studied and optimized. It indicated that the optimum conditions were as fol- lows： the ratio of brewer＇s spent grain and wheat bran was 8 ： 2, the ratio of water to material was 1.5：1, the content of the ammonium sulfate was 3%, and the fermenta- tion time was 4 d, respectively. Under the above-mentioned conditions, the filter paper activity （FPA） and carboxymethyl cellulase activity （CMCA） can reach 72. 611 8and 692. 170 0 nkat, respectively.
出处
《青岛科技大学学报（自然科学版）》
CAS
北大核心
2012年第3期263-268,共6页
Journal of Qingdao University of Science and Technology:Natural Science Edition
基金
山东省科技攻关项目(2009GG10007001)
山东省自然科学杰出青年基金项目(JQ200904)
关键词
啤酒糟
黑曲霉
固态发酵
纤维素酶
brewer＇s spent grain
Aspergillus niger
solid-state fermentation
cellulase
