摘要
目的 :探讨依赖还原型辅酶I/II醌氧化还原酶基因cDNA60 9位C→T点突变所致的基因多态性是否与肺癌遗传多态性有关。方法 :采用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)的分析方法分析了 95例肺癌病人与 136例健康成人对照组NQO1的基因多态性。结果 :T等位基因频率在肺癌组和对照组分别为5 4%、43% ,两组有显著差异 (P =0 0 2 4)。基因型分布在肺癌组和对照组之间差异显著 (p =0 0 0 8)。T/C和T/T基因型携带者患肺癌的危险性分别是野生型纯合子 (C/C)的 2 949倍 (95 %可信限 :1 42 6～ 6 0 96 )与 3 10 6倍(95 %可信限 :1 2 84～ 9 5 14)。结论 :本研究结果提示NQO1基因cDNA60 9突变T等位基因可能是肺癌发生的危险性因素 ,与肺癌的遗传易感性有关。
Objectives:To investigate whether lung cancer is associated with genetic polymorphism of NQO1 gene (NAD(P) H:quinone oxidoreductase gene)caused by the point mutation of cDNA 609 C to T Methods:The polymorphism of NQO1 gene was analyzed in 95 patients with lung cancer and 136 unrelated healthy adult individuals by utilizing polymerase chain reaction restriction fragment length polymorphism (PCR RFLP) assay Results:In this study, the frequencies of T allele were 54%and 43%in the lung cancer group and the health control group respectively, which was significantly different between the two groups(P=0 024) The allelic frequency of the mutant T allele was significantly higher in the lung cancer patients as compared to controls There was a significant difference in the overall genotypical distribution between the patients and the controls (P=0 008) The risk of suffering from lung cancer was increased 2 949 times in the individuals with T/C genotype (95%CI:1 426～6 096) and 3 01 times in the individuals of T/T genotype of NQO1 gene (95%CI:1 284～9 514) Conclusion:This study suggested that the cDNA609 T allele of NQO1 gene might be the risk factor of lung cancer, which could be associated with genetic susceptibility of lung cancer
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2000年第5期450-452,共3页
Chinese Journal of Cancer
