摘要
目的:探讨依赖还原型辅酶Ⅰ/Ⅱ:醌氧化还原酶(NQO1)基因多态性与大肠癌遗传易感性之间的相关性。方法:采用聚合酶链反应—限制性片段长度多态性(PCR-RFLP)基因型分析技术对101例大肠癌患者及103例对照者NQO1cDNA609位点多态性进行测定。结果:基因型频率分布在大肠癌组与对照组差异显著(χ2=9.52,P<0.01),T/T基因型携带者大肠癌患病危险性是野生纯合型(C/C)的3.56倍(OR=3.56,95%CI为1.58～7.96)。NQO1T等位基因及C等位基因频率在大肠癌组与对照组分别为42.1%、57.3%,57.9%、42.7%,T等位基因频率两组有显著差异(χ2=9.43,P<0.01),T等位基因携带者患大肠癌的危险性是C等位基因携带者的1.85倍,(OR=1.85,95%CI为1.25～2.73)。结论:NQO1在大肠癌的形成过程中起一定的保护作用,而NQO1cDNA609位点T等位基因可能是大肠癌发生的危险因素,NQO1基因的多态性与生活特征共同决定个体大肠癌的患病风险。
Objective: To investigate the association between colorectal cancer genetic susceptibility and the genetic polymorphism of NQO1 NAD(P)H: quinone oxidoreductase 1 gene caused by the point mutation of cDNA 609 C to T. Methods: The polymorphism of NQO1 gene was analyzed in 101 colorectal cancer patients and 103 adult individuals by utilizing PCR-RFLP (polymerase chain reaction restriction fragment length polymorphism ) genotyping assay technique. Results: There is a significance difference in the frequencies of the mutated NQO1 genotype (T/T): 0.327 for cancer patients and 0.175 for control individuals (χ2=9.52, P<0.01). The individuals with the T/T genotype had a increasing cancer risk than individuals with C/C genotypes; the OR for T/T versus C/C was 3.56 (95% CI, 1.58-7.96). The frequency distribution of T allele and C allele was 42.1% and 57.3% in cases and 57.9% and 42.7% in controls, respectively (χ2=9.42, P<0.01). The individuals with the T allele had a slight increasing cancer risk than individuals with C allele; the OR for T versus C was 1.85 (95% CI, 1.25-2.73). Conclusion: This study may suggest a protect role of the NQO1 wild type(C/C) in the colorectal tumorgenesis and the cDNA 609T allele of NQO1gene may be the risk factor of colorectal cancer. There is also that the individual's overall susceptibility to colorectal cancer is a consequence of both an inheritable trait in NQO1 metabolism and a behavioral trait in individual living characteristics.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2004年第2期89-91,共3页
Chinese Journal of Clinical Oncology
关键词
依赖还原型辅酶Ⅰ/Ⅱ
醌氧化还原酶
大肠癌遗传易感性
基因
态性
NQo1NAD(P)H: quinone oxidoreductase 1 Colorectal cancer Susceptibility to cancer Genetic polymorphism
