摘要
目的观察温郁金对胃癌细胞抑制作用和MMP-2、CD44v6蛋白表达的影响,探讨其抗胃癌细胞转移的作用机制。方法以SGC-7901胃癌细胞株建立胃癌裸鼠原位移植瘤模型,将裸鼠随机分为对照组(0.9%氯化钠溶液)及实验组(温郁金水煎剂)。观察裸小鼠胃癌种植后肿瘤生长及转移灶情况,用免疫组化法检测2组肿瘤组织中MMP-2和CD44v6蛋白的表达。结果 2组荷瘤鼠胃壁均有肿瘤生长,荷瘤率100%,对照组瘤重(4.09±1.17)g,实验组瘤重(2.73±0.92)g(与对照组比较P<0.05),抑瘤率为33.25%;实验组肝、腹腔和淋巴结转移较生理盐水对照组明显减少(P<0.05);实验组瘤体中MMP-2和CD44v6的表达明显下调。结论温郁金具有抑制人胃癌裸鼠原位移植瘤生长和转移的作用,其作用机制可能与下调MMP-2和CD44v6的表达有关。
Objective To study the inhibitory effect of common turmeric and the expression of MMP-2 and CD44v6 proteins in human gastric SGC-7901cell,explore the possible mechanisms on gastric cancer metastasis.Method Established nude mouse orthotopic transplantation mode of SGC-7901 and then randomly divided the nude mouse into control group and common turmeric group.The tumor growth and metastasis were observed,the expression of MMP-2 and CD44v6 proteins in the tumor tissue were detected by immunohistochemistry.Results The rate of successfully orthotopic transplantation was 100%.The weight of the tumors in common turmeric group was（2.73±0.92）g,in control group was（4.09±1.17）g,there was statistical significance between the two group（P〈0.05）,The inhibitary rate of common turmeric group was 33.25%.The metastasis of cavitas peritonealis,liver and lymph node in common turmeric group were significantly lower than those of control group（P〈0.05）.Meanwhile,we found that the positive rates of MMP-2 and CD44v6 expression in the common turmeric group were obviously lower than that in control group（P〈0.05）.Conclusions Common turmeric can inhibit gastric cancer growth and metastasis in orthotopic transplantation model of nude mice,the mechanism might be related to down-regulation of MMP-2 and CD44v6 expression.
出处
《医学动物防制》
2012年第6期620-622,共3页
Journal of Medical Pest Control
