摘要
目的：探讨多病毒感染时细胞免疫和ＮＫ，ＬＡＫ细胞活性的变化及其意义。方法：以１种或多种病毒抗原，或ＩｇＭ抗体阳性的住院患儿６１例为观察对象，其中单病毒组３８例，多病毒组２３例，另设对照组３０例，采用ＥＬＩＳＡ法测定柯萨奇病毒（ＣＶＢ）抗原和ＩｇＭ抗体、ＥＢ病毒（ＥＢＶ）ＩｇＭ，巨细胞病毒（ＣＶＭ）ＩｇＭ、乙肝病毒（ＨＢＶ）血清标志物５项，单克隆抗体的间接ＡＢＣ免疫组化法测定Ｔ细胞亚群；
Objective:To investigate the changes of T cell immunity,NK and LAK cell activities and their significance in multiviral infection. Methods:Sixty-one cases with positive virus antigen or IgM antibody of single or several virus (38 single virus, 23 several virus ) and 30 controls were enrolled in the study. We detected CVB-Ag, CVB-IgM, EBV-IgM, CMV-IgM and HBV serumal markers using ELISA method, T lymphocyte subpopulation using OKT method, NK and LAK cell activities using H-TdR method. Results: (1) CD4/CD8 ratio, NK cell activity and LAK cell activity were obviously decreased in early viral infection stage and the level of CD8 obviously increased (P<0. 01 ); (2) Both LAK cell activity and the CD4/CD8 ratio improved in convalesence of single viral infection (P<0. 01,P<0. 05 ); (3) In convalescence of multiviral infection, NK and LAK cell activities decreased more markedly than the activities in early viral infection stage (P<0. 05, P<O. 01). Conclusion: In single viral infection the body immune system can self-regulate against viral infection, but the immune system is damaged very much in multiviral infection, so more attention should be paid to it in clinical.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
2000年第4期340-342,共3页
Academic Journal of Second Military Medical University
基金
国家自然科学基金资助项目!(39770318)
关键词
多病毒感染
T细胞亚群
儿童
NK细胞
LAK细胞
multiviral infection
T lympholyte subpopulation
killer cells,natural
killer cells, lymphokineactirated
