摘要
目的：系统探讨柯萨奇病毒Ｂ（ＣＶＢ）感染血症的机体免疫特征及干扰素应用的评价。方法：采用ＥＬＩＳＡ法测定ＣＶＢＡｇ和ＣＶＢＩｇＭ；采用单克隆抗体标记的间接ＡＢＣ免疫组化法测定Ｔ细胞亚群；３ＨＴｄＲ标记法测定ＮＫ细胞和ＬＡＫ细胞活性。结果：（１）ＣＶＢ感染机体ＮＫ细胞活性．ＬＡＫ细胞活性和ＣＤ４／ＣＤ８比值明显低于对照组；（２）机体ＮＫ和ＬＡＫ细胞活性轻中度降低，ＣＶＢＡｇ可自然清除或应用α干扰素清除；若ＮＫ和ＬＡＫ细胞活性严重降低，则ＣＶＢＡｇ清除慢，应用α干扰素也不能清除抗原。结论：α干扰素在ＣＶＢ感染中有应用价值。
Objective: To systemically study the influence of state of cell immunity on therapeutical efficacy of interferon α in Coxsackievirus B infection. Methods:We detected NK,LAKcell activity using 3H TdR method,T lymphocyte subpopulation using OKT method, and CVB Ag and CVB IgM using ELISA method. Results: (1)NK cell activity, LAK cell activity, and CD4/CD8 ratio all decreased remarkably in CVB infection;(2) CVB Ag could be eliminated spontaneously or by interferon α; when the NK and LAK cell activity decreased severely, CVB Ag was eliminated slowly, but it could not be eliminated by interferon α. Conclusion: This results suggest that interferon α has therapeutical efficacy in CVB infection.
出处
《第二军医大学学报》
CAS
CSCD
北大核心
1997年第2期170-172,共3页
Academic Journal of Second Military Medical University
