摘要
目的 :探讨用中药海参猴桃液 (SSAP)辅以基因重组人白细胞介素 2 (rIL -2 )体外培养扩增、激活免疫杀伤细胞LAK的机理 ,观察SSAP辅以少量rIL -2能否提高及在多大程度上提高LAK细胞的杀瘤活性。方法 :通过提取健康供血者的外周血单个核细胞作为待培养细胞 ,设空白对照组 (加等量生理盐水 )、阳性对照组 (1 0 0 0U/mlrIL -2 1ml)、实验 1组 (1 0 0 0U/mlrIL -2 0 .5ml +30 0 0 μg/mlSSAP 1ml)、实验 2组 (1 0 0 0U/mlrIL -20 .1ml+30 0 0 μg/mlSSAP 1ml) ,4组在不同条件下培养LAK细胞 ,分别用H -TdR释放法检定其体外对人肝癌BEF -740 4杀伤活性。结果 :30 0 0 μg/ml的SSAP +1 0 0 0U/mlrIL -20 .1ml在培养第 3天便能测出杀伤活性 ,第 9天达峰值 [杀瘤率 (75 .5± 1 3.6 ) % ],杀瘤活性与 1 0倍量rIL -2单独诱导激活的LAK细胞杀瘤活性相近 [杀瘤率 (92 .9± 2 1 .3) % ],两组比较无显著性差异 (P >0 .0 5 ) ,而且杀瘤活性持续 1 8天。结论 :SSAP辅以少量的rIL -2能大量扩增激活LAK细胞 ,增强其对肿瘤细胞的杀伤力 ,更重要的意义在于获得的LAK细胞杀伤力强大而持久 ,同时可减少rIL
Purpose:?To study the possibility and the effects of Haishen Houtao Ye(SSAP) as well as a small dosage of rIL-2 on increasing and activating the immune killer cell LAK and observe whether and to which degree they can enhance the activity of the cell LAK in its ability to kill cancer cells. Methods:?The single nucleocell from peripheral blood of healthy blood donor was applied as the cultural cell. All the cells were divided into 4 groups, the control group with the same amount of normal saline, the positive control group with 1 000U/ml rIL-1/ml, the experiment group I with 1 000U/ml rIL-2?0.5ml+3 000μg/ml SSAP 1ml and the experiment group II with 1 000U/ml rIL-2?0.1ml+3 000μg/ml SSAP 1ml. The cells LAK of these 4 groups were cultured under different conditions and H-TdR method was used to determine their effects in vitro on the killing activity to human liver cancer BEF-7404 respectively. Results:?On the 3rd day of the culture , the killing activity was found and proved in both the experi.ment group I and II and their peak value was reached on the 9th day (75.5± 13.6% of the cancer cell-killing rate) with the very similar cancer cell-killing rate to that of the cell LAK induced only by 10 times of rIL-2( 92.9± 21.3% of the cancer cell-killing rate). There was no significant difference in these two groups( P >0.05). The activity lasted 18 days. Conclusions:?SSAP added with a small dosage of rIL-2 can greatly increase and activate the cell LAK and enhance its ability to kill cancer cells. Furthermore, the acquired?LAK? cells can keep longer and stronger killing e^ffects on cancer cells with less application of rIL-2.
出处
《广西中医药》
2000年第2期46-48,59,共4页
Guangxi Journal of Traditional Chinese Medicine
基金
广西壮族自治区卫生厅资助课题!(No.9402)
