摘要
采用酶解法提取仿刺参消化道多糖并进行初步性质测定。方法:通过木瓜蛋白酶酶解获得粗多糖,并综合考虑酶解时间、温度、加酶量的影响,获得优化的酶解条件;采用三氯醋酸法除蛋白,Sephacryl S-400凝胶柱层析进行纯化,并测定其相对分子质量和硫酸基含量。结果:酶解最佳条件为加酶量10400U/g,酶解温度55℃,酶解时间6h,多糖得率8.11‰;纯化多糖为组分单一的酸性多糖,分子质量约25kD,硫酸基含量约为9.29%。
The enzymatic hydrolysis of Apostichopus japonicus alimental tract with papain was carried out for polysaccharide extraction,and the obtained polysaccharides were preliminarily characterized. Three hydrolysis parameters including hydroly-sis time and temperature as well as enzyme amount were optimized. The relative molecular mass and sulfate group content of the purified product of the crude extract obtained after deproteinization by Sevag method and subsequent Sephacryl S-400 gel filtration chromatography were measured. Enzyme amount of 10400 U/g,hydrolysis temperature of 55 ℃and hydrolysis time of 6 h were found optimal,and the polysaccharide yield was 8.1‰ under these hydrolysis conditions. After Sephacryl S-400 column purification,an acidic polysaccharide with single composition was obtained,and its relative molecular mass might be 25 kD and its sulfate group content was approximately 9.29%.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第20期226-229,共4页
Food Science
基金
国家海洋局海洋公益性行业重大科研专项(200805031)
国家海洋局海洋经济规划科技推进平台与运作项目(国海科字([2007]219))
关键词
仿刺参消化道多糖
分离
纯化
Apostichopus japonicus alimental tract polysaccharide
extraction
purification
