摘要
以卷丹百合(Lilium lancifolum)珠芽鳞片为试材,探讨了不同消毒处理、不同部位珠芽鳞片外植体、激素配组对卷丹百合鳞茎不定芽及愈伤组织诱导的影响。试验结果表明:80%次氯酸钠处理20min对珠芽鳞片污染率及成活率综合作用效果最佳;诱导卷丹珠芽鳞片不定芽的最佳培养基为MS+6-BA 1.0mg·L-1+NAA 0.5mg·L-1;诱导珠芽鳞片愈伤组织最佳培养基为MS+6-BA 1.5mg·L-1+NAA 0.5mg·L-1。
The key factor that affecting sterilizing and differentiation of bulblets of Lilium lancifolum were studied in this paper. The results tested by orthogonal design showed that the comprehensive effect of the best treatment was 80%NaCIO for 20 minutes to deal with pearl bud scales, the rate of pollution and the survival rate. The best formula for primary culture of the hulblet scales was MS＋ 6-BA 1.0mg· L-1 ＋ NAA 0.5 mg· L-1. The best formula for secondary culture of the bulblet callus was MS-/6-BA 1.5mg· L-1 1 Jr NAA 0.5mg· L-1.
出处
《山西农业大学学报（自然科学版）》
CAS
2012年第2期146-149,共4页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
山西省科技重大攻关项目(20080311010-1)
关键词
卷丹百合
珠芽
组织培养
Lilium lanclfolium
Bulblets
Tissue culture
