摘要
目的对烧伤后早期肠内特异抗白念菌IgA变化及其白念菌粘附肠粘膜的关系进行观察。方法利用无特殊病原菌(SPF)的小鼠定植白念菌模型 ,致以20%TBSAⅢ度烧伤。ELISA法检测伤前及伤后1、2、3d的肠内特异抗白念菌IgA水平 ;同时选择培养并计数盲肠及小肠粘膜表面粘附的白念菌。结果伤前组特异IgA为(69.37±25.37)U/mg,伤后1d为(48.84±26.47)U/mg,2d明显降为(38.22±23.57)U/mg,3d为(52.64±37.35)U/mg;肠内白念菌总量伤后第1天最高 ;伤后第2、3天粘附菌量明显升高。结论特异IgA下降与肠粘膜表面粘附菌量增多密切相关。
Objective To study the relationship of the decline of specific IgA antibody in the intestine to the adherence of Candida albicans to intestinal mucosa in the early stage after severe burns in mice. Methods SPF mice were inoculated with Candida albicans 14 days before the animals were inflicted with 20% TBSA full thickness burns. The level of specific IgA against C.albicans in the intestine was determined before and 1, 2 and 3 days after burn injury. C.albicans clonies on the mucous membrane of the cecum and small intestine was selectively cultured and counted. Results The decrease of the level of specific IgA was positively correlated to the increase of the number of C.albicans colonies on the intestinal mucosa. Conclusion The decline of specific IgA showed strong correlation to the increased amount of candida adhence to intestinal mucosa.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第4期338-340,共3页
Journal of Third Military Medical University
基金
全军"九五"攻关项目!(96L042)
