摘要
目的：探讨重组人白细胞介素－４（ｒｈＩＬ－４）对放射损伤合并烧伤时肠粘膜免疫功能障碍的调节作用。方法：采用小鼠１５％的Ⅲ度烧伤、８Ｇｙ放射损伤及两伤复合的动物模型，用免疫酶联吸附、免疫组化和细菌培养等方法，观察了ｒｈＩＬ－４对肠道粘膜免疫的调控作用。结果：ｒｈＩＬ－４能增加小肠固有层中分泌ＩｇＡ的浆细胞数量，提高肠粘液中ｓＩｇＡ的含量，降低肠源性感染的发生。结论：ｒｈＩＬ－４对恢复肠道粘膜免疫功能，降低肠源性感染的发生是有效的。
Objective: To investigate the regulative effects of recombinant human interleukin 4 (rhIL 4) on intestinal mucosal immune dysfunction after radiation, burn and combined radiation burn injuries. Methods: After the models of 15% TBSA full thickness skin burn, 8Gy radiation and combined radiation burn injuries were established in mice, the regulative effects of rhIL 4 on intestinal mucosal immune dysfunction were observed with immunohistochemistry, ELISA and bacterial culture. Results: rhIL 4 increased the number of IgA secreting plasma cells in the lamina propria and quantity of mucous sIgA and reduced the incidence of enterogenous infection. Conclusion: rhIL 4 is effective for promoting the recovery of mucosal immune function and decreasing the incidence of enterogenous infection.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1999年第1期1-4,共4页
Journal of Third Military Medical University
基金
国家自然科学基金
全军"九五"指令性课题
关键词
小肠粘膜
放射损伤
烧伤
免疫功能
rhIL-4
combined radiation burn injury
intestinal mucosal immune
recombinant human interleukin 4
sIgA
plasma cell
enterogenous infection
