摘要
目的:检测食管癌组织中P-糖蛋白(P-glycopro-tein,PgP)、胸苷酸磷酸化酶(thymidine phosp-horylase,TP)、谷胱甘肽-S-转移酶(glutathione S-transferase,GST-π)、多药耐药相关蛋白(multidrug resistance-associated protein,MRP)表达情况,探讨其与食管癌化疗敏感性的关系.方法:选取我院食管癌患者58例,用免疫组织化学方法检测食管癌组织中多药耐药相关蛋白PgP、TP、GST-π、MRP蛋白的表达情况;行双铂方案新辅助化疗,分析化疗疗效与PgP、TP、GST-π、MRP蛋白表达差异的关系.结果:PgP阳性颗粒分布于胞膜和胞浆;TP阳性颗粒分布于细胞胞浆和胞核;GST-π阳性颗粒分布于胞浆;MRP阳性颗粒分布于胞膜和胞浆,他们在食管癌组织中都存在一定的表达率,表达率分别为41.37%、32.76%、20.69%和37.93%.化疗有效组患者与化疗无效组患者相比较,食管癌组织中PgP、GSH和MRP的阳性表达率低,TP阳性表达率高,两组之间有显著性差异.结论:食管癌组织中PgP、TP、GST-π、MRP存在一定的表达率;PgP、GST-π、MRP表达率低而TP表达率高,化疗治疗的效果好.
AIM： To study the relationship between ex- pression of P-glycoprotein （PgP）, thymidine phosphorylase （TP）, glutathione S-transferase-π （GST-π）, and multidrug resistance-associated protein （MRP） and response to chemotherapy in patients with esophageal cancer. METHODS： Fifty-eight patients gastroscopically and pathologically diagnosed with esophageal cancer were included in this study. Esophageal cancer samples collected from these patients were used to detect the expression of PgP, TP, GST-π, and MRP by immunohistochemistry. All the patients underwent neoadjuvant chemotherapy with double-platinum regimen. Response to chemotherapy and expression of PgP, TP, GST-π, and MRP was analyzed. RESULTS： PgP-positive particles were distrib- uted in the membrane and cytoplasm, TP-posi- tive particles in the cytoplasm and nucleus, GST-π-positive particles in the cytoplasm, and MRP- positive particles in the cytoplasm. The positive rates of PgP, TP, GST-π, and MRP expression in esophageal cancer were 41.37%, 32.76%, 20.69% and 37.93%, respectively. Compared to patients not responding to chemotherapy, the positive rates of PgP, GSH and MRP expression were sig- nificantly lower and that of TP was significantly higher in patients having response to chemo- therapy. CONCLUSION： Low expression of PgP, GST-π, and MRP and high expression of TP are condu- cive to chemotherapy in patients with esopha- geal cancer.
出处
《世界华人消化杂志》
CAS
北大核心
2012年第19期1768-1772,共5页
World Chinese Journal of Digestology
