摘要
为筛选出适宜开展能力验证的药物品种的储存条件和运输条件,最大限度消除外因对检测结果的影响,保证结果的可靠性,以草鱼肌肉为研究对象,开展了五氯酚钠残留检测质量控制技术研究。通过分析五氯酚钠在低、中、高3个浓度(3.0、5.0、50μg/kg)水平和3种贮藏条件(常温25℃,冷藏4℃和冷冻-18℃)下随时间的降解规律,不同浓度的五氯酚钠因贮藏条件的不同,均有不同程度的降解,且降解速率各有不同。草鱼在常温条件下,降解速率表现为高浓度的降解速率>中浓度的降解速率>低浓度的降解速率;在冷藏条件下,降解速率均表现为中浓度的降解速率>低浓度的降解速率>高浓度的降解速率;在冷冻条件下,降解速率均表现为高浓度的降解速率>低浓度的降解速率>中浓度的降解速率。不同贮藏条件对五氯酚钠加标样品的影响不同,受酶作用的影响,短时间内保存五氯酚钠加标样品可以在常温进行,便于尽快进行检测;如样品不能尽快安排检测(时间超过24h),样品需用冷冻方法进行保存,这样有利于保存样品的原有属性,避免对检测结果造成大的影响。
Quality control technology of sodium pentachlorophenol residues in muscle of grass carpwas carried out to find out the appropriate storage and transportation conditions for drug verification, with an aim to minimize the impacts of external factors on the test results and to ensure the reliability of the test results. Grass carp was chosen as representive substrates to analyze the degradation of sodium pentachlorophenol over time in 3 different concentration levels （3.0, 5.0, 50 μg/kg） and 3 different storage conditions （ normal room temperature 25 ℃, cold storage 4℃ and freezing - 18 ℃ ）. The results showed that under different storage conditions, the degradation of sodium pentachlorophenol in varying concentrations varied and the degradation rate also varied. When grass carp was experimented in normal room temperature, it was found that： degradation under high concentration 〉 degradation under medium concentration 〉 degradation under low concentration. And under cold storage, the result was different with degradation under medium concentration 〉 degradation under low concentration 〉 degradation under high concentration. And under freezing conditions, the result was different with degradation under medium concentration 〉 degradation under low concentration 〉 degradation under high concentration. It was concluded that different storage conditions had different effects on the spiked sodium pentachlorophenol samples. By the impact of enzyme, short - term preservation of sodium pentachlorophenol spiked samples could be stored at normal room temperature, which was also easy to conduct detection soon. If the samples couldn＇ t be arranged to be detected within 24 hours, the samples should be preserved by freezing conditions, which was a good preservation keeping its original properties and also a good way to avoid great impacts on the test results.
出处
《中国渔业质量与标准》
2012年第2期63-69,共7页
Chinese Fishery Quality and Standards
基金
罗非鱼现代农业产业技术体系建设专项"罗非鱼产业技术体系"(2060302-425-02)
中央级公益性科研院所基本科研业务费"几种水产品药物残留检测质量控制技术研究"(2010C010)
关键词
五氯酚钠
残留
降解
草鱼
sodium pentachlorophenol
residues
degradation
grass carp
