摘要
对52株木霉菌株进行尼罗红染色、菌丝油脂提取和纤维素酶、木质素降解酶活力测定,发现一株哈茨木霉(Trichodermaharzianum)菌株Q2-37为最优菌株。该菌株干菌丝中的油脂含量为(20.5±0.36)%。以玉米秸秆粉为唯一碳源的培养基质上培养Q2-37,每利用100g玉米秸秆粉可以产生(1.188±0.028)g油脂。对受试菌株提取得到的油脂含量进行分析发现,木霉干菌丝的油脂含量范围在(12.6±0.31)%^(20.5±0.36)%。该研究表明,木霉利用植物秸秆生产油脂为生物柴油提供廉价原料是具有潜力的。
Trichoderma harzianum Q2-37 was found to be the best one among 52 Trichoderma isolates when tested on their lipid productivity by Nile red staining and mycelial biomass extracting,cellulose and lignase activity assay.The dry mycelial biomass of Q2-37 contained lipid by（20.5±0.36）% of the total.Q2-37 was cultured on corn straw meals as the sole carbon source and produced 1.188g lipid from 100g of the straw.The oil content of dry hyphae of the tested strains ranges from（20.5±0.36）% to（12.6±0.31）%.The result showed that Trichoderma isolates could be promising to produce cheaper bio-diesel materials by decomposing plant stubbles.
出处
《中国酿造》
CAS
2012年第5期154-158,共5页
China Brewing
基金
科技部863计划(2011AA10A205)
