摘要
目的探讨神经肽Y(NPY)和5-羟色胺(5-HT)对腹泻型肠易激综合征(D-IBS)发病机制的影响,为从脑肠轴方面分析研究D-IBS的发生机制提供理论依据。方法采用母乳分离+醋酸刺激+四肢束缚制作D-IBS大鼠模型,通过免疫组化法检测大鼠结肠组织中5-HT的蛋白表达水平,采用实时荧光定量RT-PCR检测大鼠下丘脑和结肠组织中NPY mRNA的表达。结果与正常组相比,NPY mRNA表达在肠易激综合征模型大鼠结肠和下丘脑组织均降低(P<0.05)。5-HT蛋白表达在模型大鼠结肠中升高(P<0.05)。结论脑肠肽NPY和5-HT可能参与D-IBS脑肠轴异常机制的调节。
Aim To investigate expression of neu-ropeptide Y（NPY） and 5-hydroxy tryptamine （5-HT） in diarrhea-predominant irritable bowel syndrome （D- IBS） and provide theoretical basis for mechanism of D- IBS from the aspect of brain-gut axis. Method A rat model of D-IBS was established with the use of separation of breast milk ＋ stimulation of acetic acid ＋ con- straint of the four limbs. Expression of 5-HT protein was detected in colon of rat with immunohistochemistry and expression of NPY mRNA was detected in colon and hypothalamus of rat with real-time fluorescence quantitative reverse transcription polymerase chain reaction （ RT-PCR ）. Result Compared with normal group, expression of NPY mRNA were significantly de- creased in colon and hypothalamus of D-IBS model group （P 〈 0.05）. In addition, 5-HT protein expression was markedly increased in colon of D-IBS model group （P 〈 0. 05 ）. Conclusion NPY and 5-HT may be involved in regulatory mechanism of D-IBS.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2012年第7期916-920,共5页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81073160)
广东省自然科学基金资助项目(No 10151040701000041)
广州市科技局重大科技生物专项资助项目(No 2008AJ-E4101-6)
广东省科技厅科技资助项目(No 2010B030700065)
关键词
神经肽Y
5-羟色胺
腹泻型肠易激综合征
脑肠轴
实时荧光定量PCR
下丘脑
结肠
neuropeptider Y
5-hydroxy tryptamine
diarrhea-predominant irritable bowed syndrome
braingut axis
reverse transcription polymerase chain reaction
hypothalamus
colon
