摘要
目的:研究皖南蝮蛇毒抑瘤组分Ⅰ(Agkis-trodon halys venom antitumor component Ⅰ,AHVAC-Ⅰ)对人胃癌细胞SGC-7901荷瘤鼠的体内抑制作用及瘤细胞增殖活性变化,并初步探讨其作用机制。方法:取生长状态良好的浓度为1×107个/mL的SGC-7901人胃癌细胞悬液0.1mL接种于裸小鼠右侧腋窝皮下,待其成瘤后随机分为模型组、紫杉醇阳性对照组、AH-VAC-Ⅰ高、中、低剂量组,隔日连续给药5次,定期观察肿瘤生长情况,测量肿瘤体积,绘制肿瘤生长曲线并计算抑瘤率,HE染色观察瘤体形态学变化,免疫组化SP法检测Ki-67增殖指数,原位末端标记技术(TUNEL)法检测细胞凋亡指数。结果:AHVAC-Ⅰ在1.0mg/kg剂量时对SGC-7901细胞有抑制作用,抑瘤率达42.2%;在3.0mg/kg剂量时有显著抑制作用,抑瘤率达78.5%。实验组肿瘤内Ki-67阳性细胞数量下降,凋亡细胞明显增多。结论:AHVAC-Ⅰ能明显抑制裸鼠体内SGC-7901人胃癌细胞的生长,其作用机制可能与诱导胃癌细胞发生凋亡及抑制肿瘤细胞增殖有关。
ABSTRACT AIM： To investigate the in vivo tumor-inhibitory effects of snake venom antttu mot component I（AHVAC-I） from Agkistrodon halys living in south Anhui province on the tumor-bearing mice with human gastric cancer cell SGC-7901 and activities of the cell prolifera- tion as well as its mechanisms. METHODS： Ceil suspension of 0.1 mL was obtained from the well-grown cell SGC-7901 by cell count of 1 ×10^7/mL and incubated subcutaneously into the nude mice via right axillary fossa. After tumor formation, the mice were randomized into groups of model controls, positive paclitaxiel controls, AHVAC-I of low, medium and low dosage in which the animals were managed with AHVAC-I every other day for 5 consecutive times. The tumor growth and its sizes were ob- served and measured for mapping the growth curve and calculating the inhibitory rate. HE staining was performed to examine the morpho-logic change of tumors, and streptavidin-peroxi- dase （SP） immunohistochemical technique was used to detect the proliferation index of Ki-67 and TUNEL method was for examination of the apoptotic index. RESULTS：AHVAC-I at dose of 1.0 mg/kg produced inhibitory effects on SGC- 7901 with inhibition rate of 42.2%, and inhibi- tory effects were significant at close of 3.0 mg/kg with a rate of 78.5%. Ki-67 protein was reduced in the positive cells of treatment groups, leading to increased apoptosis of tumor cells. CONCLUSION= AHVAC-I can effectively inhibit the in vivo growth of gastric cell SGC 7901 in nude mice, and the possible mechanisms may be associated with the induced apoptosis and sup- pressed proliferation of the gastric tumor cells.
出处
《中国临床药理学与治疗学》
CAS
CSCD
2012年第5期491-496,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
安徽省科技厅基金资助项目(10021303009)
芜湖市科技局科研基金项目(09卫生类1-1)
