摘要
[目的]利用呼吸道合胞病毒（RSV）A、B亚型特异性cDNA探针，检测和鉴别病毒分离株或临床标本，分析广州地区分离株RSV亚型。[方法]利用RSV两个亚型病毒C蛋白胞外区段编码基因的cDNA克隆片段，随机引物法标记，合成地高辛甙元标记RSV亚型特异的探针，检测12株广州地区分离株和120份临床标本。[结果]两型探针均可检出1pg由RSV纯病毒颗粒提取的RNA，两亚型间无交叉反应；12株分离株全部为A亚型，120份临床标本检出9例A亚型与病毒分离结果一致，未检出B亚型病毒。[结论]用非同位毒标记RSV亚型特异性探针，具有很高的特异性和敏感性，其操作快速、简便、安全，可在临床应用。临床初步应用结果表明，广州地区临床分离株以A亚型为主。
[Objective] To analysis the subgroups of the respiratory syncytial virus (RSV) in Guangzhou. [Methods] The cDNA of the RNA sequences which encode the extracellular domains of G proteins of the two RSV subgroups were used as probes to differentiate the A and B subgroups of isolated RSV strains, and to detected the clinic samples. The nonradioative dig-DNA nabelling system and detecting sysem were used. [Results] The probes are of high specifity and sensitivety. 12 RSV isolated strains and 120 clinic samples were tested by these probes, All the isolated strains and the positive strains from clinic samples are of A subgroup, no B subgroup strain was found. [Conclusions] The RSV A subgroup strains in Guangzhou is dominat.
出处
《中国儿童保健杂志》
CAS
2000年第2期88-89,共2页
Chinese Journal of Child Health Care
